Molecular cloning and characterization of the yeast RAD10 gene and expression of RAD10 protein in E. coli.

W. A. Weiss, E. C. Friedberg

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A plasmid designated pNF101 was isolated by transforming rad10 mutants with a yeast genomic library and screening transformed cells for enhanced resistance to killing by u.v. radiation. Plasmid pNF101 fully complements the u.v. sensitivity of rad10 mutant strains and was shown to contain the RAD10 gene by genetic analysis of integrant strains. The nucleotide sequence of the RAD10 gene was determined. The coding region consists of 195 codons and could encode a polypeptide of calculated mol. wt. 22 616 daltons. RAD10 protein expressed in Escherichia coli maxicells has a mol. wt of approximately 30 kd measured by gel electrophoresis. The RAD10 gene was localized to chromosome XIII of Saccharomyces cerevisiae by hybridization of the cloned gene to yeast chromosomes resolved by electrophoresis, and by genetic analysis.

Original languageEnglish (US)
Pages (from-to)1575-1582
Number of pages8
JournalEMBO Journal
Volume4
Issue number6
StatePublished - Jun 1985

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Cloning
Escherichia coli Proteins
Molecular Cloning
Yeast
Escherichia coli
Genes
Yeasts
Gene Expression
Chromosomes
Electrophoresis
Proteins
Plasmids
Genomic Library
Codon
Saccharomyces cerevisiae
Screening
Nucleotides
Gels
Radiation
Peptides

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Molecular cloning and characterization of the yeast RAD10 gene and expression of RAD10 protein in E. coli. / Weiss, W. A.; Friedberg, E. C.

In: EMBO Journal, Vol. 4, No. 6, 06.1985, p. 1575-1582.

Research output: Contribution to journalArticle

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