Molecular cloning and developmental expression of the caveolin gene family in the amphibian Xenopus laevis

Babak Razani, David S. Park, Yuko Miyanaga, Ashwini Ghatpande, Justin Cohen, Xiao Bo Wang, Philipp E. Scherer, Todd Evans, Michael P. Lisanti

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Caveolae are ∼50-100 nm invaginations of the plasma membrane thought to form as a result of a local accumulation of cholesterol, sphingolipids, and a unique family of three proteins known as the caveolins: Cav-1, -2, and -3. Here, we report the identification, sequence, and developmental expression of the three caveolin genes in the amphibian Xenopus laevis. Sequence comparisons show that Xenopus Cav-1, -2, and -3 are ∼80, 64, and 45% identical, respectively, to their counterparts in humans. Furthermore, Northern blotting experiments demonstrate that the Xenopus caveolins have tissue-specific expression profiles consistent with those previously reported in adult mammals. In the adult frog, Xenopus Cav-1 and Cav-2 are most abundantly expressed in the fat body and the lungs, while Xenopus Cav-3 is primarily expressed in muscle tissue types (heart and skeletal muscle). However, our temporal and spatial analyses of these expression patterns during embryogenesis reveal several novel features, with possible relevance to developmental signaling. Transcripts encoding Xenopus Cav-1 and -2 first appear in the notochord of neurula stage embryos, which represents a key signaling tissue. In contrast, Xenopus Cav-3 shows a highly specific punctate expression pattern in the embryonic epidermis, similar to previous patterns implicated in Notch signaling. These findings are in striking contrast to their steady-state expression patterns in the adult frog. Taken together, our results show that the Xenopus caveolin gene family is present and differentially expressed in both embryonic and adult tissues. This report is the first detailed study of caveolin gene expression in a developing embryo.

Original languageEnglish (US)
Pages (from-to)7914-7924
Number of pages11
JournalBiochemistry
Volume41
Issue number25
DOIs
StatePublished - Jun 25 2002

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Caveolins
Cloning
Xenopus laevis
Molecular Cloning
Amphibians
Xenopus
Genes
Gene Expression
Tissue
Muscle
Anura
Sphingolipids
Mammals
Embryonic Structures
Cell membranes
Spatio-Temporal Analysis
Gene expression
Notochord
Caveolin 1
Caveolae

ASJC Scopus subject areas

  • Biochemistry

Cite this

Razani, B., Park, D. S., Miyanaga, Y., Ghatpande, A., Cohen, J., Wang, X. B., ... Lisanti, M. P. (2002). Molecular cloning and developmental expression of the caveolin gene family in the amphibian Xenopus laevis. Biochemistry, 41(25), 7914-7924. https://doi.org/10.1021/bi020043n

Molecular cloning and developmental expression of the caveolin gene family in the amphibian Xenopus laevis. / Razani, Babak; Park, David S.; Miyanaga, Yuko; Ghatpande, Ashwini; Cohen, Justin; Wang, Xiao Bo; Scherer, Philipp E.; Evans, Todd; Lisanti, Michael P.

In: Biochemistry, Vol. 41, No. 25, 25.06.2002, p. 7914-7924.

Research output: Contribution to journalArticle

Razani, B, Park, DS, Miyanaga, Y, Ghatpande, A, Cohen, J, Wang, XB, Scherer, PE, Evans, T & Lisanti, MP 2002, 'Molecular cloning and developmental expression of the caveolin gene family in the amphibian Xenopus laevis', Biochemistry, vol. 41, no. 25, pp. 7914-7924. https://doi.org/10.1021/bi020043n
Razani B, Park DS, Miyanaga Y, Ghatpande A, Cohen J, Wang XB et al. Molecular cloning and developmental expression of the caveolin gene family in the amphibian Xenopus laevis. Biochemistry. 2002 Jun 25;41(25):7914-7924. https://doi.org/10.1021/bi020043n
Razani, Babak ; Park, David S. ; Miyanaga, Yuko ; Ghatpande, Ashwini ; Cohen, Justin ; Wang, Xiao Bo ; Scherer, Philipp E. ; Evans, Todd ; Lisanti, Michael P. / Molecular cloning and developmental expression of the caveolin gene family in the amphibian Xenopus laevis. In: Biochemistry. 2002 ; Vol. 41, No. 25. pp. 7914-7924.
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