Molecular cloning and functional identification of a plant ornithine decarboxylase cDNA

Anthony J. Michael, Judith M. Furze, Michael J C Rhodes, Daniel Burtin

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

A cDNA for a plant ornithine decarboxylase (ODC), a key enzyme in putrescine and polyamine biosynthesis, has been isolated from root cultures of the solanaceous plant Datura stramonium. Reverse transcription-PCR employing degenerate oligonucleotide primers representing conserved motifs from other eukaryotic ODCs was used to isolate the cDNA. The longest open reading frame potentially encodes a peptide of 431 amino acids and exhibits similarity to other eukaryotic ODCs, prokaryotic and eukaryotic arginine decarboxylases (ADCs), prokaryotic meso-diaminopimelate decarboxylases and the product of the tabA gene of Pseudomonas syringae cv. tabaci. Residues involved at the active site of the mouse ODC are conserved in the plant enzyme, The plant ODC does not possess the C-terminal extension found in the mammalian enzyme, implicated in rapid turnover of the protein, suggesting that the plant ODC may have a longer half-life. Expression of the plant ODC in Escherichia coli and demonstration of ODC activity confirmed that the cDNA encodes an active ODC enzyme. This is the first description of the primary structure of a eukaryotic ODC isolated from an organism where the alternative ADC route to putrescine is present.

Original languageEnglish (US)
Pages (from-to)241-248
Number of pages8
JournalBiochemical Journal
Volume314
Issue number1
StatePublished - Feb 15 1996

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Ornithine Decarboxylase
Cloning
Molecular Cloning
Complementary DNA
Putrescine
Enzymes
Datura stramonium
Pseudomonas syringae
Plant Proteins
DNA Primers
Carboxy-Lyases
Biosynthesis
Polyamines
Transcription
Escherichia coli
Open Reading Frames
Reverse Transcription
Half-Life
Catalytic Domain
Demonstrations

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molecular cloning and functional identification of a plant ornithine decarboxylase cDNA. / Michael, Anthony J.; Furze, Judith M.; Rhodes, Michael J C; Burtin, Daniel.

In: Biochemical Journal, Vol. 314, No. 1, 15.02.1996, p. 241-248.

Research output: Contribution to journalArticle

Michael, AJ, Furze, JM, Rhodes, MJC & Burtin, D 1996, 'Molecular cloning and functional identification of a plant ornithine decarboxylase cDNA', Biochemical Journal, vol. 314, no. 1, pp. 241-248.
Michael, Anthony J. ; Furze, Judith M. ; Rhodes, Michael J C ; Burtin, Daniel. / Molecular cloning and functional identification of a plant ornithine decarboxylase cDNA. In: Biochemical Journal. 1996 ; Vol. 314, No. 1. pp. 241-248.
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