Molecular cloning and nucleotide sequences of cDNA clones of sheep and goat adrenocortical cytochromes P450scc (CYP11A1)

Eisaku Okuyama, Tomoko Okazaki, Aizo Furukawa, Ru Feng Wu, Yoshiyuki Ichikawa

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We isolated cDNA clones of the mRNAs for cytochromes P450scc (CYP11A1) from sheep and goat adrenocortices using the RT-PCR method. We determined the complete nucleotide sequences of the coding and 3′-flanking regions of these cDNA clones. The results confirmed the amino terminal sequence of the sheep cytochrome P450scc reported previously [Miyatake et al., Biochim. Biophys. Acta 1215, 176-182 (1994)]. On the basis of comparison of the deduced amino acid sequences of various animals and rainbow trout cytochromes P450scc, and other mitochondrial cytochrome P450 isozymes, we discussed the substrate-associated and adreno-ferredoxin-binding region of cytochrome P450scc.

Original languageEnglish (US)
Pages (from-to)179-185
Number of pages7
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume57
Issue number3-4
DOIs
StatePublished - Feb 1996

Fingerprint

Cholesterol Side-Chain Cleavage Enzyme
Cloning
Molecular Cloning
Goats
Sheep
Nucleotides
Complementary DNA
Clone Cells
3' Flanking Region
Ferredoxins
Oncorhynchus mykiss
Cytochrome P-450 Enzyme System
Isoenzymes
Amino Acid Sequence
Animals
Amino Acids
Polymerase Chain Reaction
Messenger RNA
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Molecular cloning and nucleotide sequences of cDNA clones of sheep and goat adrenocortical cytochromes P450scc (CYP11A1). / Okuyama, Eisaku; Okazaki, Tomoko; Furukawa, Aizo; Wu, Ru Feng; Ichikawa, Yoshiyuki.

In: Journal of Steroid Biochemistry and Molecular Biology, Vol. 57, No. 3-4, 02.1996, p. 179-185.

Research output: Contribution to journalArticle

@article{a939070ecd74433c9021e49a9cc84723,
title = "Molecular cloning and nucleotide sequences of cDNA clones of sheep and goat adrenocortical cytochromes P450scc (CYP11A1)",
abstract = "We isolated cDNA clones of the mRNAs for cytochromes P450scc (CYP11A1) from sheep and goat adrenocortices using the RT-PCR method. We determined the complete nucleotide sequences of the coding and 3′-flanking regions of these cDNA clones. The results confirmed the amino terminal sequence of the sheep cytochrome P450scc reported previously [Miyatake et al., Biochim. Biophys. Acta 1215, 176-182 (1994)]. On the basis of comparison of the deduced amino acid sequences of various animals and rainbow trout cytochromes P450scc, and other mitochondrial cytochrome P450 isozymes, we discussed the substrate-associated and adreno-ferredoxin-binding region of cytochrome P450scc.",
author = "Eisaku Okuyama and Tomoko Okazaki and Aizo Furukawa and Wu, {Ru Feng} and Yoshiyuki Ichikawa",
year = "1996",
month = "2",
doi = "10.1016/0960-0760(95)00263-4",
language = "English (US)",
volume = "57",
pages = "179--185",
journal = "Journal of Steroid Biochemistry and Molecular Biology",
issn = "0960-0760",
publisher = "Elsevier Limited",
number = "3-4",

}

TY - JOUR

T1 - Molecular cloning and nucleotide sequences of cDNA clones of sheep and goat adrenocortical cytochromes P450scc (CYP11A1)

AU - Okuyama, Eisaku

AU - Okazaki, Tomoko

AU - Furukawa, Aizo

AU - Wu, Ru Feng

AU - Ichikawa, Yoshiyuki

PY - 1996/2

Y1 - 1996/2

N2 - We isolated cDNA clones of the mRNAs for cytochromes P450scc (CYP11A1) from sheep and goat adrenocortices using the RT-PCR method. We determined the complete nucleotide sequences of the coding and 3′-flanking regions of these cDNA clones. The results confirmed the amino terminal sequence of the sheep cytochrome P450scc reported previously [Miyatake et al., Biochim. Biophys. Acta 1215, 176-182 (1994)]. On the basis of comparison of the deduced amino acid sequences of various animals and rainbow trout cytochromes P450scc, and other mitochondrial cytochrome P450 isozymes, we discussed the substrate-associated and adreno-ferredoxin-binding region of cytochrome P450scc.

AB - We isolated cDNA clones of the mRNAs for cytochromes P450scc (CYP11A1) from sheep and goat adrenocortices using the RT-PCR method. We determined the complete nucleotide sequences of the coding and 3′-flanking regions of these cDNA clones. The results confirmed the amino terminal sequence of the sheep cytochrome P450scc reported previously [Miyatake et al., Biochim. Biophys. Acta 1215, 176-182 (1994)]. On the basis of comparison of the deduced amino acid sequences of various animals and rainbow trout cytochromes P450scc, and other mitochondrial cytochrome P450 isozymes, we discussed the substrate-associated and adreno-ferredoxin-binding region of cytochrome P450scc.

UR - http://www.scopus.com/inward/record.url?scp=0029936365&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029936365&partnerID=8YFLogxK

U2 - 10.1016/0960-0760(95)00263-4

DO - 10.1016/0960-0760(95)00263-4

M3 - Article

VL - 57

SP - 179

EP - 185

JO - Journal of Steroid Biochemistry and Molecular Biology

JF - Journal of Steroid Biochemistry and Molecular Biology

SN - 0960-0760

IS - 3-4

ER -