Molecular cloning and primary structure of Thermoactinomyces vulgaris carboxypeptidase T A metalloenzyme endowed with dual substrate specificity

Sergey V. Smulevitch, Andrey L. Osterman, Olga V. Galperina, Mikhail V. Matz, Olga P. Zagnitko, Rafail M. Kadyrov, Iraida A. Tsaplina, Nikolai V. Grishin, Galina G. Chestukhina, Valentin M. Stepanov

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

A gene coding for an extracellular Zn-carboxypeptidase of Thermoactinomyces vulgaris has been cloned and sequenced (EMBL X56901). This enzyme named carboxypeptidase T reveals simultaneously both types of substrate specificity characteristic of mammalian carboxypeptidases A and B. The carboxypeptidase T gene is primarily expressed in E. coli as a non-active preproenzyme with an additional 98 amino acid residues at the N-terminus. Primary structure alignment or mature carboxypeptidase T and mammalian metallocarboxypeptidases demonstrated 25-30% overall identity but a full preservation of presumed catalytically important residues. These observations imply a basic uniformity of the general catalytic mechanism for enzymes of that class produced by evolutionarily remote organisms.

Original languageEnglish (US)
Pages (from-to)75-78
Number of pages4
JournalFEBS Letters
Volume291
Issue number1
DOIs
StatePublished - Oct 7 1991

Keywords

  • Carboxypeptidase T
  • Extracellular metalloenzyme
  • Genomic library
  • Preproenzyme
  • Primary structure
  • Thermoactinomyces vulgaris

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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    Smulevitch, S. V., Osterman, A. L., Galperina, O. V., Matz, M. V., Zagnitko, O. P., Kadyrov, R. M., Tsaplina, I. A., Grishin, N. V., Chestukhina, G. G., & Stepanov, V. M. (1991). Molecular cloning and primary structure of Thermoactinomyces vulgaris carboxypeptidase T A metalloenzyme endowed with dual substrate specificity. FEBS Letters, 291(1), 75-78. https://doi.org/10.1016/0014-5793(91)81107-J