Molecular-cloning and tissue-specific expression of fructose 6-phosphate 2-kinase:fructose 2,6-bisphosphatase of rat brain

F. Watanabe, A. Sakai, E. Furuya, K. Uyeda

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

A cDNA clone (3591 base pairs) encoding an isozyme of Fructose 6-phosphate, 2-kinase:Fructose 2,6-bisphosphatase was isolated from a rat brain cDNA library. The 5′ sequence of this clone (1241 base pairs) was identical to that of the heart type isozyme cDNA (except for 7 base pair mismatches), but the 3′ nucleotide sequence (2024 base pairs) was completely different. Its deduced amino acid sequence showed that the enzyme lacked a regulatory domain which contained phosphorylation sites for protein kinase A and C. The results of Northern blotting and polymerase chain reaction demonstrated that the mRNA, 7.4 kilobases long, was expressed also in heart, testis, liver, and skeletal muscle.

Original languageEnglish (US)
Pages (from-to)335-340
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume198
Issue number1
DOIs
StatePublished - Jan 15 1994

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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