Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes

J. A. Trapani, J. L. Klein, P. C. White, B. Dupont

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

A cDNA clone encoding a human serine esterase gene was isolated from a library constructed from poly(A)+ RNA of allogeneically stimulated, interleukin 2-expanded peripheral blood mononuclear cells. The clone, designated HSE26.1, represents a full-length copy of a 0.9-kilobase mRNA present in human cytotoxic cells but absent from a wide variety of noncytotoxic cell lines. Clone HSE26.1 contains an 892-base-pair sequence, including a single 741-base-pair open reading frame encoding a putative 247-residue polypeptide. The first 20 amino acids of the polypeptide form a leader sequence. The mature protein is predicted to have an unglycosylated M(r) of ≃26,000 and contains a single potential site for N-linked glycosylation. The nucleotide and predicted amino acid sequences of clone HSE26.1 are homologous with all murine and human serine esterases cloned thus far but are most similar to mouse granzyme B (70% nucleotide and 68% amino acid identity). HSE26.1 protein is expressed weakly in unstimulated peripheral blood mononuclear cells but is strongly induced within 6-hr incubation in medium containing phytohemagglutinin. The data suggest that the protein encoded by HSE26.1 plays a role in cell-mediated cytotoxicity.

Original languageEnglish (US)
Pages (from-to)6924-6928
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number18
StatePublished - 1988

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Molecular Cloning
Clone Cells
Lymphocytes
Base Pairing
Genes
Blood Cells
Nucleotides
Amino Acids
Granzymes
Messenger RNA
Peptides
Proteins
Phytohemagglutinins
Glycosylation
Open Reading Frames
Libraries
Interleukin-2
Amino Acid Sequence
Complementary DNA
Cell Line

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes. / Trapani, J. A.; Klein, J. L.; White, P. C.; Dupont, B.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 18, 1988, p. 6924-6928.

Research output: Contribution to journalArticle

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