Abstract
Chemokine receptors mediate leukocyte migration into secondary lymphoid tissues and localization to peripheral inflammation sites. We describe full-length cDNA sequences of bovine chemokine receptors CCR5, CCR7, CXCR3 and CXCR5 and transcript expression by WC1+ γδ T cells, a unique cell population with proinflammatory characteristics that comprises a large proportion of mononuclear cells in young ruminants. Bovine chemokine sequences were more similar to those of humans than were murine sequences to humans', ranging from 84% to 91%. Transcript analysis showed that antigen stimulation of WC1+ γδ T cells induced IFN-γ production and substantially increased CCR5 and CXCR3 expression when compared with freshly isolated (ex vivo) cells. CCR7 transcripts were minimally expressed in ex vivo and proliferating WC1+ γδ T cells and CXCR5 expression was negligible. These results confirm the proinflammatory nature of WC1+ γδ T cells is reflected by its chemokine receptor expression and suggest WC1+ γδ T cells are unlikely to transit through secondary lymphoid tissues.
Original language | English (US) |
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Pages (from-to) | 87-102 |
Number of pages | 16 |
Journal | Developmental and Comparative Immunology |
Volume | 31 |
Issue number | 1 |
DOIs | |
State | Published - 2007 |
Keywords
- CCR5
- CCR7
- CXCR3
- CXCR5
- WC1
- γδ T cells
ASJC Scopus subject areas
- Immunology
- Developmental Biology