Molecular cloning of bovine chemokine receptors and expression by WC1+ γδ T cells

Seth L. Blumerman, Fei Wang, Carolyn T A Herzig, Cynthia L. Baldwin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Chemokine receptors mediate leukocyte migration into secondary lymphoid tissues and localization to peripheral inflammation sites. We describe full-length cDNA sequences of bovine chemokine receptors CCR5, CCR7, CXCR3 and CXCR5 and transcript expression by WC1+ γδ T cells, a unique cell population with proinflammatory characteristics that comprises a large proportion of mononuclear cells in young ruminants. Bovine chemokine sequences were more similar to those of humans than were murine sequences to humans', ranging from 84% to 91%. Transcript analysis showed that antigen stimulation of WC1+ γδ T cells induced IFN-γ production and substantially increased CCR5 and CXCR3 expression when compared with freshly isolated (ex vivo) cells. CCR7 transcripts were minimally expressed in ex vivo and proliferating WC1+ γδ T cells and CXCR5 expression was negligible. These results confirm the proinflammatory nature of WC1+ γδ T cells is reflected by its chemokine receptor expression and suggest WC1+ γδ T cells are unlikely to transit through secondary lymphoid tissues.

Original languageEnglish (US)
Pages (from-to)87-102
Number of pages16
JournalDevelopmental and Comparative Immunology
Volume31
Issue number1
DOIs
StatePublished - Jan 1 2007

Fingerprint

Chemokine Receptors
Molecular Cloning
T-Lymphocytes
Lymphoid Tissue
Ruminants
Chemokines
Leukocytes
Complementary DNA
Inflammation
Antigens
Population

Keywords

  • γδ T cells
  • CCR5
  • CCR7
  • CXCR3
  • CXCR5
  • WC1

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

Cite this

Molecular cloning of bovine chemokine receptors and expression by WC1+ γδ T cells. / Blumerman, Seth L.; Wang, Fei; Herzig, Carolyn T A; Baldwin, Cynthia L.

In: Developmental and Comparative Immunology, Vol. 31, No. 1, 01.01.2007, p. 87-102.

Research output: Contribution to journalArticle

Blumerman, Seth L. ; Wang, Fei ; Herzig, Carolyn T A ; Baldwin, Cynthia L. / Molecular cloning of bovine chemokine receptors and expression by WC1+ γδ T cells. In: Developmental and Comparative Immunology. 2007 ; Vol. 31, No. 1. pp. 87-102.
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