Molecular Cloning of Bovine LDL Receptor cDNAs

D. W. Russell; T. Yamamoto

doi:10.1016/0076-6879(86)28113-6

Molecular Cloning of Bovine LDL Receptor cDNAs

D. W. Russell, T. Yamamoto

Molecular Genetics

Research output: Contribution to journal › Article › peer-review

Abstract

Using methods described above, a partial cDNA clone for the bovine LDL receptor has been isolated. DNA sequence analysis and Northern blotting experiments are used to confirm the identity of pLDLR-1. Further DNA sequence analysis of pLDLR-1 reveals that the partial cDNA insert encodes 264 amino acids corresponding to the carboxy-terminal 25% of the bovine LDL receptor.²⁴ Antipeptide antibodies directed against regions of the predicted protein sequence specifically recognize the purified bovine receptor. These findings provide an independent confirmation of the identity of pLDLR-1.

Original language	English (US)
Pages (from-to)	895-909
Number of pages	15
Journal	Methods in Enzymology
Volume	128
Issue number	C
DOIs	https://doi.org/10.1016/0076-6879(86)28113-6
State	Published - Jan 1 1986

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1016/0076-6879(86)28113-6

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abstract = "Using methods described above, a partial cDNA clone for the bovine LDL receptor has been isolated. DNA sequence analysis and Northern blotting experiments are used to confirm the identity of pLDLR-1. Further DNA sequence analysis of pLDLR-1 reveals that the partial cDNA insert encodes 264 amino acids corresponding to the carboxy-terminal 25% of the bovine LDL receptor.24 Antipeptide antibodies directed against regions of the predicted protein sequence specifically recognize the purified bovine receptor. These findings provide an independent confirmation of the identity of pLDLR-1.",

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year = "1986",

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AB - Using methods described above, a partial cDNA clone for the bovine LDL receptor has been isolated. DNA sequence analysis and Northern blotting experiments are used to confirm the identity of pLDLR-1. Further DNA sequence analysis of pLDLR-1 reveals that the partial cDNA insert encodes 264 amino acids corresponding to the carboxy-terminal 25% of the bovine LDL receptor.24 Antipeptide antibodies directed against regions of the predicted protein sequence specifically recognize the purified bovine receptor. These findings provide an independent confirmation of the identity of pLDLR-1.

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Molecular Cloning of Bovine LDL Receptor cDNAs

Abstract

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