Molecular cloning of bovine LDL receptor cDNAs

D. W. Russell, T. Yamamoto

Research output: Contribution to journalArticle

Abstract

Using methods described above, a partial cDNA clone for the bovine LDL receptor has been isolated. DNA sequence analysis and Northern blotting experiments are used to confirm the identity of pLDLR-1. Further DNA sequence analysis of pLDLR-1 reveals that the partial cDNA insert encodes 264 amino acids corresponding to the carboxy-terminal 25% of the bovine LDL receptor. Antipeptide antibodies directed against regions of the predicted protein sequence specifically recognize the purified bovine receptor. These findings provide an independent confirmation of the identity of pLDLR-1.

Original languageEnglish (US)
Pages (from-to)895-909
Number of pages15
JournalMethods in Enzymology
VolumeVOL. 128
StatePublished - 1986

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LDL Receptors
Cloning
DNA sequences
Molecular Cloning
Complementary DNA
DNA Sequence Analysis
Amino Acids
Northern Blotting
Antibodies
Clone Cells
Proteins
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Molecular cloning of bovine LDL receptor cDNAs. / Russell, D. W.; Yamamoto, T.

In: Methods in Enzymology, Vol. VOL. 128, 1986, p. 895-909.

Research output: Contribution to journalArticle

Russell, DW & Yamamoto, T 1986, 'Molecular cloning of bovine LDL receptor cDNAs', Methods in Enzymology, vol. VOL. 128, pp. 895-909.
Russell, D. W. ; Yamamoto, T. / Molecular cloning of bovine LDL receptor cDNAs. In: Methods in Enzymology. 1986 ; Vol. VOL. 128. pp. 895-909.
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