Molecular cloning of complementary DNA for the alpha subunit of the G protein that stimulates adenylate cyclase

Bruce A. Harris, Janet D. Robishaw, Susanne M. Mumby, Alfred G. Gilman

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A complementary DNA clone encoding the a subunit of the adenylate cyclase stimulatory G protein (Gs) was isolated and identified. A bovine brain complementary DNA library was screened with an oligonucleotide probe derived from amino acid sequence common to known G proteins. The only clone that was obtained with this probe has a complementary DNA insert of approximately 1670 base pairs. An antibody to a peptide synthesized according to deduced amino acid sequence reacts specifically with the a subunit of Gs. In addition, RNA that hybridizes with probes made from the clone is detected in wild-type S49 cells; however, cyc- S49 cells, which are deficient in G activity, are devoid of this messenger RNA.

Original languageEnglish (US)
Pages (from-to)1274-1277
Number of pages4
Issue number4719
StatePublished - Jan 1 1985


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