Molecular cloning of complementary DNA for the alpha subunit of the G protein that stimulates adenylate cyclase

Bruce A. Harris, Janet D. Robishaw, Susanne M. Mumby, Alfred G. Gilman

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

A complementary DNA clone encoding the a subunit of the adenylate cyclase stimulatory G protein (Gs) was isolated and identified. A bovine brain complementary DNA library was screened with an oligonucleotide probe derived from amino acid sequence common to known G proteins. The only clone that was obtained with this probe has a complementary DNA insert of approximately 1670 base pairs. An antibody to a peptide synthesized according to deduced amino acid sequence reacts specifically with the a subunit of Gs. In addition, RNA that hybridizes with probes made from the clone is detected in wild-type S49 cells; however, cyc- S49 cells, which are deficient in G activity, are devoid of this messenger RNA.

Original languageEnglish (US)
Pages (from-to)1274-1277
Number of pages4
JournalScience
Volume229
Issue number4719
StatePublished - 1985

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GTP-Binding Protein alpha Subunits
Molecular Cloning
Adenylyl Cyclases
Complementary DNA
Clone Cells
Amino Acid Sequence
Gs GTP-Binding Protein alpha Subunits
Oligonucleotide Probes
Gene Library
GTP-Binding Proteins
Base Pairing
RNA
Messenger RNA
Peptides
Antibodies
Brain

ASJC Scopus subject areas

  • General

Cite this

Harris, B. A., Robishaw, J. D., Mumby, S. M., & Gilman, A. G. (1985). Molecular cloning of complementary DNA for the alpha subunit of the G protein that stimulates adenylate cyclase. Science, 229(4719), 1274-1277.

Molecular cloning of complementary DNA for the alpha subunit of the G protein that stimulates adenylate cyclase. / Harris, Bruce A.; Robishaw, Janet D.; Mumby, Susanne M.; Gilman, Alfred G.

In: Science, Vol. 229, No. 4719, 1985, p. 1274-1277.

Research output: Contribution to journalArticle

Harris, BA, Robishaw, JD, Mumby, SM & Gilman, AG 1985, 'Molecular cloning of complementary DNA for the alpha subunit of the G protein that stimulates adenylate cyclase', Science, vol. 229, no. 4719, pp. 1274-1277.
Harris, Bruce A. ; Robishaw, Janet D. ; Mumby, Susanne M. ; Gilman, Alfred G. / Molecular cloning of complementary DNA for the alpha subunit of the G protein that stimulates adenylate cyclase. In: Science. 1985 ; Vol. 229, No. 4719. pp. 1274-1277.
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