Molecular cloning of the mature E1b-β subunit of human branched-chain α-keto acid dehydrogenase complex

Jacinta L. Chuang, Rody P. Cox, David T. Chuang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have isolated a cDNA encoding the E1b-β subunit of the human branched-chain α-keto acid dehydrogenase complex. The human E1b-β cDNA is 1401 base pairs in length. It encodes the entire mature E1b-β subunit consisting of 342 amino acid residues, and a mitochondrial targeting presequence of 31 residues. The calculated molecular mass of the mature human E1b-β subunit is 37 851 Da, and the calculated isoelectric point is pH 5.18. A hydropathy plot shows that the human E1b-β subunit is highly hydrophobic. Northern blot analysis shows that the human E1b-β mRNA is approximately 1.4 kb in size. It is present at the normal level in fibroblasts from two unrelated maple syrup urine disease patients.

Original languageEnglish (US)
Pages (from-to)305-309
Number of pages5
JournalFEBS Letters
Volume262
Issue number2
DOIs
StatePublished - Mar 26 1990

Fingerprint

3-Methyl-2-Oxobutanoate Dehydrogenase (Lipoamide)
Cloning
Molecular Cloning
Complementary DNA
Molecular mass
Fibroblasts
Amino Acids
Messenger RNA
Maple Syrup Urine Disease
Isoelectric Point
Base Pairing
Northern Blotting

Keywords

  • (Human liver)
  • Amino acid sequence
  • Branched-chain E1b-β
  • cDNA
  • Nucleotide sequence

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Molecular cloning of the mature E1b-β subunit of human branched-chain α-keto acid dehydrogenase complex. / Chuang, Jacinta L.; Cox, Rody P.; Chuang, David T.

In: FEBS Letters, Vol. 262, No. 2, 26.03.1990, p. 305-309.

Research output: Contribution to journalArticle

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