Molecular cloning, pharmacological characterization, and histochemical distribution of frog vasotocin and mesotocin receptors

S. Acharjee, J. L. Do-Rego, Dayoung Oh, J. S. Moon, R. S. Ahn, K. Lee, D. G. Bai, H. Vaudry, H. B. Kwon, J. Y. Seong

Research output: Contribution to journalArticle

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Abstract

The neurohypophysial nonapeptides vasotocin (VT) and mesotocin (MT) are the amphibian counterparts of arginine vasopressin (AVP) and oxytocin (OT). We have here reported the cloning and functional characterization of the receptors for vasotocin (VTR) and mesotocin (MTR) in two species of frog, Rana catesbeiana and Rana esculenta. The frog VTR and MTR cDNAs encode proteins of 419 and 384 amino acids respectively. Frog VTR exhibits a high degree of sequence identity with the mammalian AVP-1a (V1a) receptor while the frog MTR possesses a high degree of sequence identity with the mammalian OT receptor. Activation of VTR induced both c-fos promoter- and cAMP-responsive element (CRE)-driven transcriptional activities, while activation of MTR induced c-fos promoter-driven transcriptional activity but failed to evoke CRE-driven transcriptional activity, suggesting differential G protein coupling between VTR and MTR. The VTR exhibited the highest sensitivity for VT followed by OT > AVP≈MT, whereas the MTR showed preferential ligand sensitivity for MT > OT > VT > AVP. AV1a agonist but not V2 and OT agonists substantially activated both VTR and MTR with a similar sensitivity. V1a, V2 and OT antagonists inhibited MT-induced MTR activation but not VT-induced VTR activation. In the frog brain, VTR and MTR mRNAs were found to be widely expressed in the telencephalon, diencephalon and mesencephalon, and exhibited very similar regional distribution. In the pituitary, VTR and MTR were expressed in the distal and intermediate lobes but were virtually absent in the neural lobe. Taken together, these data indicated that, although the distribution of VTR and MTR largely overlaps in the frog brain and pituitary, VT and MT may play distinct activities owing to the ligand selectivity and different signaling pathways activated by their receptors.

Original languageEnglish (US)
Pages (from-to)293-313
Number of pages21
JournalJournal of Molecular Endocrinology
Volume33
Issue number1
DOIs
StatePublished - Aug 1 2004

Fingerprint

Vasotocin
Molecular Cloning
Anura
Pharmacology
Oxytocin
Arginine Vasopressin
Rana esculenta
Oxytocin Receptors
Ligands
Posterior Pituitary Gland
Rana catesbeiana
Vasopressin Receptors
Diencephalon
Telencephalon
Brain
Amphibians
Mesencephalon
GTP-Binding Proteins
mesotocin receptor
vasotocin receptor

ASJC Scopus subject areas

  • Endocrinology

Cite this

Molecular cloning, pharmacological characterization, and histochemical distribution of frog vasotocin and mesotocin receptors. / Acharjee, S.; Do-Rego, J. L.; Oh, Dayoung; Moon, J. S.; Ahn, R. S.; Lee, K.; Bai, D. G.; Vaudry, H.; Kwon, H. B.; Seong, J. Y.

In: Journal of Molecular Endocrinology, Vol. 33, No. 1, 01.08.2004, p. 293-313.

Research output: Contribution to journalArticle

Acharjee, S. ; Do-Rego, J. L. ; Oh, Dayoung ; Moon, J. S. ; Ahn, R. S. ; Lee, K. ; Bai, D. G. ; Vaudry, H. ; Kwon, H. B. ; Seong, J. Y. / Molecular cloning, pharmacological characterization, and histochemical distribution of frog vasotocin and mesotocin receptors. In: Journal of Molecular Endocrinology. 2004 ; Vol. 33, No. 1. pp. 293-313.
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AU - Do-Rego, J. L.

AU - Oh, Dayoung

AU - Moon, J. S.

AU - Ahn, R. S.

AU - Lee, K.

AU - Bai, D. G.

AU - Vaudry, H.

AU - Kwon, H. B.

AU - Seong, J. Y.

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