Molecular complexity of Qa-2 antigens demonstrated by two-dimensional gel electrophoresis.

P. J. Robinson, I. Lefkovits, K. F. Lindahl

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Abstract

Biosynthetically labelled Qa-2 antigens were isolated from mouse spleen cells by immunoprecipitation with anti-Qa-2 antisera. When newly synthesized Qa-2 molecules from several different inbred strains were analysed by two-dimensional (2D) gel electrophoresis; four different phenotypes were observed that differed in the number of polypeptides present. The ability to distinguish Qa-2+ phenotypes was used to map the recombination points in two congenic strains, B6.Tlaa and A.Tlab. No alternative Qa-2-like polypeptides were detected in B6.K1 (Qa-2-) cells using a polyspecific rabbit antiserum against mouse class I antigens, but a new molecule was detected in BALB/cBy (Qa-2-) cells. Pulse-chase and surface-labelling experiments showed that some, but not all, of the newly synthesized Qa-2 precursor forms are processed to mature cell surface molecules.

Original languageEnglish (US)
Pages (from-to)81-87
Number of pages7
JournalJournal of Immunogenetics
Volume14
Issue number2-3
Publication statusPublished - Apr 1987

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ASJC Scopus subject areas

  • Genetics
  • Immunology

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