Molecular evolution of neuropeptide receptors with regard to maintaining high affinity to their authentic ligands

Hyun Ju Cho, Sujata Acharjee, Mi Jin Moon, Dayoung Oh, Hubert Vaudry, Hyuk Bang Kwon, Jae Young Seong

Research output: Contribution to journalShort survey

31 Citations (Scopus)

Abstract

Recently, we cloned many of the bullfrog neuropeptide G protein-coupled receptors (GPCRs), including receptors for vasotocin (VT), mesotocin, gonadotropin-releasing hormone (GnRH), neurotensin, apelin, and metastin. Bullfrog GPCRs usually have high affinity for bullfrog ligands but relatively low affinity for mammalian ligands. Reciprocally, synthetic agonists and antagonists developed based upon mammalian ligands display lower affinity at bullfrog receptors. Studies using chimeric or domain-swapped receptors indicate that the motifs responsible for differential ligand selectivity usually reside within transmembrane domain 6 (TMD6)-extracellular loop 3 (ECL3)-transmembrane domain 7 (TMD7). Triple mutation of mammalian V1aR (Phe6.51 to Tyr, Ile6.53 to Thr, and Pro7.33 to Thr) increases VT affinity but greatly reduces arginine vasopressin affinity. This binding profile is similar to that of bullfrog VT1R. Changing just three amino acids in the bullfrog GnRH receptor-1 (i.e. Ser-Gln-Ser in the ECL3) to those found in the type-I mammalian GnRH receptor (i.e. Ser-Glu-Pro) reverses GnRH selectivity. In conclusion, specific receptor motifs that govern ligand selectivity can be determined by comparative molecular analyses of GPCRs and their ligands. Such analysis provides clues for understanding how GPCRs maintain high affinity to their authentic ligands.

Original languageEnglish (US)
Pages (from-to)98-107
Number of pages10
JournalGeneral and Comparative Endocrinology
Volume153
Issue number1-3
DOIs
StatePublished - Jan 1 2007

Fingerprint

Neuropeptide Receptors
Molecular Evolution
Rana catesbeiana
Ligands
gonadotropin-releasing hormone
G-Protein-Coupled Receptors
LHRH Receptors
hormone receptors
receptors
vasotocin
Kisspeptins
Vasotocin
arginine vasopressin
Neurotensin
Arginine Vasopressin
ligands
neuropeptide receptors
Neuropeptides
Gonadotropin-Releasing Hormone
agonists

Keywords

  • Coevolution
  • G protein-coupled receptors
  • Ligand selectivity
  • Neuropeptides

ASJC Scopus subject areas

  • Endocrinology

Cite this

Molecular evolution of neuropeptide receptors with regard to maintaining high affinity to their authentic ligands. / Cho, Hyun Ju; Acharjee, Sujata; Moon, Mi Jin; Oh, Dayoung; Vaudry, Hubert; Kwon, Hyuk Bang; Seong, Jae Young.

In: General and Comparative Endocrinology, Vol. 153, No. 1-3, 01.01.2007, p. 98-107.

Research output: Contribution to journalShort survey

Cho, Hyun Ju ; Acharjee, Sujata ; Moon, Mi Jin ; Oh, Dayoung ; Vaudry, Hubert ; Kwon, Hyuk Bang ; Seong, Jae Young. / Molecular evolution of neuropeptide receptors with regard to maintaining high affinity to their authentic ligands. In: General and Comparative Endocrinology. 2007 ; Vol. 153, No. 1-3. pp. 98-107.
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