Molecular forms of atrial natriuretic peptides in dog atrium and plasma

P. Cernacek, E. Maher, J. C. Crawhall, M. Levy

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The level of immunoreactive ANP (iANP) as determined by radioimmunoassay, exhibits a fairly even distribution throughout the canine atria. However, the maximal concentration was found in the appendages of both the left and right atrium, and the level was significantly higher on the left side in all analyzed localizations. Reverse-phase HPLC of atrial extracts, coupled with RIA, revealed the presence of three iANP fractions. The dominant fraction (65% of iANP) has an estimated molecular weight 16,300, showing that it corresponds to the hormone precursor. By contrast, in the plasma extract, 92% of iANP coelutes with synthetic human ANP99-126, thus confirming the structural identity of dog and human ANP.

Original languageEnglish (US)
Pages (from-to)2533-2539
Number of pages7
JournalLife Sciences
Volume42
Issue number24
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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