Molecular forms of atrial natriuretic peptides in dog atrium and plasma

P. Cernacek; E. Maher; J. C. Crawhall; M. Levy

doi:10.1016/0024-3205(88)90353-0

Molecular forms of atrial natriuretic peptides in dog atrium and plasma

P. Cernacek, E. Maher, J. C. Crawhall, M. Levy

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6 Scopus citations

Abstract

The level of immunoreactive ANP (iANP) as determined by radioimmunoassay, exhibits a fairly even distribution throughout the canine atria. However, the maximal concentration was found in the appendages of both the left and right atrium, and the level was significantly higher on the left side in all analyzed localizations. Reverse-phase HPLC of atrial extracts, coupled with RIA, revealed the presence of three iANP fractions. The dominant fraction (65% of iANP) has an estimated molecular weight 16,300, showing that it corresponds to the hormone precursor. By contrast, in the plasma extract, 92% of iANP coelutes with synthetic human ANP_99-126, thus confirming the structural identity of dog and human ANP.

Original language	English (US)
Pages (from-to)	2533-2539
Number of pages	7
Journal	Life Sciences
Volume	42
Issue number	24
DOIs	https://doi.org/10.1016/0024-3205(88)90353-0
State	Published - 1988

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry, Genetics and Molecular Biology

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10.1016/0024-3205(88)90353-0

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@article{2213071e09ae4e15a4a38051225f9110,

title = "Molecular forms of atrial natriuretic peptides in dog atrium and plasma",

abstract = "The level of immunoreactive ANP (iANP) as determined by radioimmunoassay, exhibits a fairly even distribution throughout the canine atria. However, the maximal concentration was found in the appendages of both the left and right atrium, and the level was significantly higher on the left side in all analyzed localizations. Reverse-phase HPLC of atrial extracts, coupled with RIA, revealed the presence of three iANP fractions. The dominant fraction (65% of iANP) has an estimated molecular weight 16,300, showing that it corresponds to the hormone precursor. By contrast, in the plasma extract, 92% of iANP coelutes with synthetic human ANP99-126, thus confirming the structural identity of dog and human ANP.",

author = "P. Cernacek and E. Maher and Crawhall, {J. C.} and M. Levy",

note = "Funding Information: The authors acknowledge technical assistance by H. Champagne and P. Smolen. This work was supported by grants from Medical Research Council of Canada (VT-35271 to M.L. and from Department of Medicine, McGill University to P.C.",

year = "1988",

doi = "10.1016/0024-3205(88)90353-0",

language = "English (US)",

volume = "42",

pages = "2533--2539",

journal = "Life Sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

number = "24",

}

TY - JOUR

T1 - Molecular forms of atrial natriuretic peptides in dog atrium and plasma

AU - Cernacek, P.

AU - Maher, E.

AU - Crawhall, J. C.

AU - Levy, M.

N1 - Funding Information: The authors acknowledge technical assistance by H. Champagne and P. Smolen. This work was supported by grants from Medical Research Council of Canada (VT-35271 to M.L. and from Department of Medicine, McGill University to P.C.

PY - 1988

Y1 - 1988

N2 - The level of immunoreactive ANP (iANP) as determined by radioimmunoassay, exhibits a fairly even distribution throughout the canine atria. However, the maximal concentration was found in the appendages of both the left and right atrium, and the level was significantly higher on the left side in all analyzed localizations. Reverse-phase HPLC of atrial extracts, coupled with RIA, revealed the presence of three iANP fractions. The dominant fraction (65% of iANP) has an estimated molecular weight 16,300, showing that it corresponds to the hormone precursor. By contrast, in the plasma extract, 92% of iANP coelutes with synthetic human ANP99-126, thus confirming the structural identity of dog and human ANP.

AB - The level of immunoreactive ANP (iANP) as determined by radioimmunoassay, exhibits a fairly even distribution throughout the canine atria. However, the maximal concentration was found in the appendages of both the left and right atrium, and the level was significantly higher on the left side in all analyzed localizations. Reverse-phase HPLC of atrial extracts, coupled with RIA, revealed the presence of three iANP fractions. The dominant fraction (65% of iANP) has an estimated molecular weight 16,300, showing that it corresponds to the hormone precursor. By contrast, in the plasma extract, 92% of iANP coelutes with synthetic human ANP99-126, thus confirming the structural identity of dog and human ANP.

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DO - 10.1016/0024-3205(88)90353-0

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SP - 2533

EP - 2539

JO - Life Sciences

JF - Life Sciences

IS - 24

ER -

Molecular forms of atrial natriuretic peptides in dog atrium and plasma

Abstract

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