Molecular insights of copper sulfate exposure-induced nephrotoxicity: Involvement of oxidative and endoplasmic reticulum stress pathways

The precise pathogenic mechanism in Cu exposure-cause nephrotoxicity remains unclear. This study investigated the underlying molecular mechanism of copper sulfate (CuSO₄)-induced nephrotoxicity. Mice were treated with CuSO₄ at 50, 100, 200 mg/kg/day or co-treated with CuSO₄ (200 mg/kg/day) and 4-phenylbutyric acid (4-PBA, 100 mg/kg/day) for 28 consecutive days. HEK293 cells were treated with CuSO₄ (400 µM) with or without superoxide dismutase, catalase or 4-PBA for 24 h. Results showed that CuSO₄ exposure can cause renal dysfunction and tubular necrosis in the kidney tissues of mice. CuSO₄ exposure up-regulated the activities and mRNA expression of caspases-9 and-3 as well as the expression of glucose-regulated protein 78 (GRP78), GRP94, DNA damage-inducible gene 153 (GADD153/CHOP), caspase-12 mRNAs in the kidney tissues. Furthermore, superoxide dismutase and catalase pre-treatments partly inhibited CuSO₄-induced cytotoxicity by decreasing reactive oxygen species (ROS) production, activities of caspases-9 and-3 and DNA fragmentations in HEK293 cells. 4-PBA co-treatment significantly improved CuSO₄-induced cytotoxicity in HEK293 cells and inhibited CuSO₄ exposure-induced renal dysfunction and pathology damage in the kidney tissues. In conclusion, our results reveal that oxidative stress and endoplasmic reticulum stress contribute to CuSO₄-induced nephrotoxicity. Our study highlights that targeting endoplasmic reticulum and oxidative stress may offer an approach for Cu overload-caused nephrotoxicity.