Molecular localization of human class II MT2 and MT3 determinants

C. K. Hurley, R. C. Giles, G. Nunez, R. DeMars, L. Nadler, R. Winchester, P. Stastny, J. D. Capra

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The specificities of the monoclonal antibodies I-LR2 and 109d6, which recognize MT2- and MT3-like serologic determinants, respectively, have been confirmed by panel testing. In addition, the relationships of these antibodies to other monoclonal antibodies and alloantisera have been studied by means of cell surface fluorescence, complement-dependent cytotoxicity and immunoprecipitation. Using these monoclonal antibodies, molecules encoded by the HLA-D region have been isolated and characterized by amino acid sequencing and peptide mapping. By these criteria, the major populations of molecules bearing MT2- and MT3-like determinants are indistinguishable from DR molecules.

Original languageEnglish (US)
Pages (from-to)472-493
Number of pages22
JournalJournal of Experimental Medicine
Volume160
Issue number2
StatePublished - 1984

Fingerprint

Monoclonal Antibodies
HLA-D Antigens
Peptide Mapping
Protein Sequence Analysis
Immunoprecipitation
Fluorescence
Antibodies
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Hurley, C. K., Giles, R. C., Nunez, G., DeMars, R., Nadler, L., Winchester, R., ... Capra, J. D. (1984). Molecular localization of human class II MT2 and MT3 determinants. Journal of Experimental Medicine, 160(2), 472-493.

Molecular localization of human class II MT2 and MT3 determinants. / Hurley, C. K.; Giles, R. C.; Nunez, G.; DeMars, R.; Nadler, L.; Winchester, R.; Stastny, P.; Capra, J. D.

In: Journal of Experimental Medicine, Vol. 160, No. 2, 1984, p. 472-493.

Research output: Contribution to journalArticle

Hurley, CK, Giles, RC, Nunez, G, DeMars, R, Nadler, L, Winchester, R, Stastny, P & Capra, JD 1984, 'Molecular localization of human class II MT2 and MT3 determinants', Journal of Experimental Medicine, vol. 160, no. 2, pp. 472-493.
Hurley CK, Giles RC, Nunez G, DeMars R, Nadler L, Winchester R et al. Molecular localization of human class II MT2 and MT3 determinants. Journal of Experimental Medicine. 1984;160(2):472-493.
Hurley, C. K. ; Giles, R. C. ; Nunez, G. ; DeMars, R. ; Nadler, L. ; Winchester, R. ; Stastny, P. ; Capra, J. D. / Molecular localization of human class II MT2 and MT3 determinants. In: Journal of Experimental Medicine. 1984 ; Vol. 160, No. 2. pp. 472-493.
@article{59863d3e00d942e1be27971fbd9f9f02,
title = "Molecular localization of human class II MT2 and MT3 determinants",
abstract = "The specificities of the monoclonal antibodies I-LR2 and 109d6, which recognize MT2- and MT3-like serologic determinants, respectively, have been confirmed by panel testing. In addition, the relationships of these antibodies to other monoclonal antibodies and alloantisera have been studied by means of cell surface fluorescence, complement-dependent cytotoxicity and immunoprecipitation. Using these monoclonal antibodies, molecules encoded by the HLA-D region have been isolated and characterized by amino acid sequencing and peptide mapping. By these criteria, the major populations of molecules bearing MT2- and MT3-like determinants are indistinguishable from DR molecules.",
author = "Hurley, {C. K.} and Giles, {R. C.} and G. Nunez and R. DeMars and L. Nadler and R. Winchester and P. Stastny and Capra, {J. D.}",
year = "1984",
language = "English (US)",
volume = "160",
pages = "472--493",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "2",

}

TY - JOUR

T1 - Molecular localization of human class II MT2 and MT3 determinants

AU - Hurley, C. K.

AU - Giles, R. C.

AU - Nunez, G.

AU - DeMars, R.

AU - Nadler, L.

AU - Winchester, R.

AU - Stastny, P.

AU - Capra, J. D.

PY - 1984

Y1 - 1984

N2 - The specificities of the monoclonal antibodies I-LR2 and 109d6, which recognize MT2- and MT3-like serologic determinants, respectively, have been confirmed by panel testing. In addition, the relationships of these antibodies to other monoclonal antibodies and alloantisera have been studied by means of cell surface fluorescence, complement-dependent cytotoxicity and immunoprecipitation. Using these monoclonal antibodies, molecules encoded by the HLA-D region have been isolated and characterized by amino acid sequencing and peptide mapping. By these criteria, the major populations of molecules bearing MT2- and MT3-like determinants are indistinguishable from DR molecules.

AB - The specificities of the monoclonal antibodies I-LR2 and 109d6, which recognize MT2- and MT3-like serologic determinants, respectively, have been confirmed by panel testing. In addition, the relationships of these antibodies to other monoclonal antibodies and alloantisera have been studied by means of cell surface fluorescence, complement-dependent cytotoxicity and immunoprecipitation. Using these monoclonal antibodies, molecules encoded by the HLA-D region have been isolated and characterized by amino acid sequencing and peptide mapping. By these criteria, the major populations of molecules bearing MT2- and MT3-like determinants are indistinguishable from DR molecules.

UR - http://www.scopus.com/inward/record.url?scp=0021162375&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021162375&partnerID=8YFLogxK

M3 - Article

VL - 160

SP - 472

EP - 493

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 2

ER -