Abstract
Heat-labile enterotoxin (LT) can cause animal enteritis and diarrhea. However, the possible association of LT with embryo survival in pregnant animals and the mechanisms involved remain unknown. To investigate the effects of LT on embryo survival, we treated mouse early embryos in vitro and pregnant mice in vivo with recombinant LT. LT significantly decreased mouse embryo survival, and induced IFN-γ, IL-2 and IL-1β production in the serum and placental tissue. LT also triggered IL-1β release from LPS-primed microphages, suggesting LT can activate inflammasomes. To determine the pathway involved in LT-induced inflammasome activation, small interfering RNAs were used to knockdown NLRP3 and ASC, the key components of NLRP3 inflammasome pathway. Ablation of NLRP3 and ASC abolished LT-induced IL-1β release, confirming the involvement of NLRP3 inflammasome. By comparing two subunits of LT, only LTA but not LTB subunit was identified to activate the NLRP3 inflammasome.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 31-38 |
| Number of pages | 8 |
| Journal | Reproductive Toxicology |
| Volume | 45 |
| DOIs | |
| State | Published - Jun 1 2014 |
| Externally published | Yes |
Fingerprint
Keywords
- Cytokines
- E. coli heat-labile enterotoxin
- Embryo survival
- Inflammasome
ASJC Scopus subject areas
- Toxicology
Cite this
Molecular mechanism for the effects of E. Coli heat-labile enterotoxin on mouse embryo survival. / Li, Wenyan; Han, Dongmei; Liang, Shuang; Zhong, Zhenyu; Li, Xiujin; Wen, Jiexia; Lin, Hongyu; Wang, Liyue; Li, Xiangyun; Zhong, Xiuhui; Zhong, Fei.
In: Reproductive Toxicology, Vol. 45, 01.06.2014, p. 31-38.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular mechanism for the effects of E. Coli heat-labile enterotoxin on mouse embryo survival
AU - Li, Wenyan
AU - Han, Dongmei
AU - Liang, Shuang
AU - Zhong, Zhenyu
AU - Li, Xiujin
AU - Wen, Jiexia
AU - Lin, Hongyu
AU - Wang, Liyue
AU - Li, Xiangyun
AU - Zhong, Xiuhui
AU - Zhong, Fei
PY - 2014/6/1
Y1 - 2014/6/1
N2 - Heat-labile enterotoxin (LT) can cause animal enteritis and diarrhea. However, the possible association of LT with embryo survival in pregnant animals and the mechanisms involved remain unknown. To investigate the effects of LT on embryo survival, we treated mouse early embryos in vitro and pregnant mice in vivo with recombinant LT. LT significantly decreased mouse embryo survival, and induced IFN-γ, IL-2 and IL-1β production in the serum and placental tissue. LT also triggered IL-1β release from LPS-primed microphages, suggesting LT can activate inflammasomes. To determine the pathway involved in LT-induced inflammasome activation, small interfering RNAs were used to knockdown NLRP3 and ASC, the key components of NLRP3 inflammasome pathway. Ablation of NLRP3 and ASC abolished LT-induced IL-1β release, confirming the involvement of NLRP3 inflammasome. By comparing two subunits of LT, only LTA but not LTB subunit was identified to activate the NLRP3 inflammasome.
AB - Heat-labile enterotoxin (LT) can cause animal enteritis and diarrhea. However, the possible association of LT with embryo survival in pregnant animals and the mechanisms involved remain unknown. To investigate the effects of LT on embryo survival, we treated mouse early embryos in vitro and pregnant mice in vivo with recombinant LT. LT significantly decreased mouse embryo survival, and induced IFN-γ, IL-2 and IL-1β production in the serum and placental tissue. LT also triggered IL-1β release from LPS-primed microphages, suggesting LT can activate inflammasomes. To determine the pathway involved in LT-induced inflammasome activation, small interfering RNAs were used to knockdown NLRP3 and ASC, the key components of NLRP3 inflammasome pathway. Ablation of NLRP3 and ASC abolished LT-induced IL-1β release, confirming the involvement of NLRP3 inflammasome. By comparing two subunits of LT, only LTA but not LTB subunit was identified to activate the NLRP3 inflammasome.
KW - Cytokines
KW - E. coli heat-labile enterotoxin
KW - Embryo survival
KW - Inflammasome
UR - http://www.scopus.com/inward/record.url?scp=84893470131&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84893470131&partnerID=8YFLogxK
U2 - 10.1016/j.reprotox.2014.01.005
DO - 10.1016/j.reprotox.2014.01.005
M3 - Article
C2 - 24440567
AN - SCOPUS:84893470131
VL - 45
SP - 31
EP - 38
JO - Reproductigve Toxicoloy
JF - Reproductigve Toxicoloy
SN - 0890-6238
ER -