TY - JOUR
T1 - Molecular ordering of the cell death pathway
T2 - Bcl-2 and Bcl-xL function upstream of the ced-3-like apoptotic proteases
AU - Chinnaiyan, Arul M.
AU - Orth, Kim
AU - O'Rourke, Karen
AU - Duan, Hangjun
AU - Poirier, Guy G.
AU - Dixit, Vishva M.
PY - 1996/3/1
Y1 - 1996/3/1
N2 - Genetic analyses of Caenorhabditis elegans has identified three genes that function in the regulation of nematode cell death. Mammalian homologs of two of these genes, ced-9 and ced-3, have been identified and comprise proteins belonging to the Bcl-2 and ICE families, respectively. To date, it is unclear where the negative regulators, ced-9 and bcl-2, function relative to the death effectors, ced-3 and the mammalian ced-3 homologs, respectively. Here, the molecular order of the cell death pathway is defined. Our results establish that Bcl-2 and Bcl-xL function upstream of two members of the ICE/ CED-3 family of cysteine proteases, Yama (CPP32/apopain) and ICE-LAPS (Mch3).
AB - Genetic analyses of Caenorhabditis elegans has identified three genes that function in the regulation of nematode cell death. Mammalian homologs of two of these genes, ced-9 and ced-3, have been identified and comprise proteins belonging to the Bcl-2 and ICE families, respectively. To date, it is unclear where the negative regulators, ced-9 and bcl-2, function relative to the death effectors, ced-3 and the mammalian ced-3 homologs, respectively. Here, the molecular order of the cell death pathway is defined. Our results establish that Bcl-2 and Bcl-xL function upstream of two members of the ICE/ CED-3 family of cysteine proteases, Yama (CPP32/apopain) and ICE-LAPS (Mch3).
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U2 - 10.1074/jbc.271.9.4573
DO - 10.1074/jbc.271.9.4573
M3 - Article
C2 - 8617712
AN - SCOPUS:0029912189
SN - 0021-9258
VL - 271
SP - 4573
EP - 4576
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 9
ER -