Molecular pathology analyses of two fatal human infections of avian influenza A(H7N9) virus

Yanling Feng, Lvyin Hu, Shuihua Lu, Qingguo Chen, Ye Zheng, Dong Zeng, Jun Zhang, Anli Zhang, Liang Chen, Yunwen Hu, Zhiyong Zhang

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Results : Apart from the typical diffuse alveolar damage and hyaline membrane observed in severe influenza infection, we detected H7N9 RNA and massive intrapulmonary production of IP-10 and IL-6 mRNA using in situ hybridisation. Hyperplasia of type II pneumocytes was observed by H&E staining and immunohistochemistry. Proliferating macrophages and clustered neutrophils in the infected lungs were observed, whereas T lymphocytes, especially CD4T helper cells, were markedly depleted. No obvious complement deposition was found in lung tissues.

Conclusions : Our findings suggest that H7N9 influenza virus induced an immunological response towards overt pulmonary inflammation and systemic lymphopenia which led to intense alveolar damage and respiratory failure.

Aims : To investigate the histopathological manifestations of two fatal cases of H7N9 influenza A virus infection.

Methods : Pulmonary and hepatic specimens from two fatal cases of H7N9 influenza virus infection were examined using H&E staining. Additionally, in situ hybridisation was performed with probes (ViewRNA) targeting H7N9 RNA and IP-10, interleukin (IL)-6 mRNA. The distribution of surfactant protein A (SP-A), surfactant protein B (SP-B), CD3, CD4, CD8, CD68 and C4d were determined with immunohistochemistry.

Original languageEnglish (US)
Pages (from-to)57-63
Number of pages7
JournalJournal of clinical pathology
Volume68
Issue number1
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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