Molecular relationships of Helicobacter pylori strains in a family with gastroduodenal disease

Stephan Miehlke, Robert M. Genta, David Y. Graham, Mae F. Go

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Objective: Few studies have examined the genetic relationships of Helicobacter pylori strains affecting family members. Our aim was to do so. Methods: We characterized H. pylori isolates obtained from members of a single family presenting with various gastroduodenal diseases to examine H. pylori bacterial genetic similarity. Endoscopic evaluation with gastric mapping was performed on each individual to establish clinical and histological disease. Genomic DNA extracted from each H. pylori isolate was used to generate DNA fingerprints for each strain by REP-PCR. vacA genotypes and cagA presence were established by PCR. Results: Gastrointestinal diseases among the five members of this family included gastric adenocarcinoma in a 52-yr-old man (index patient), gastric MALT-lymphoma in the 73-yr-old mother; intestinal metaplasia (IV) and atrophic gastrifis in the 48-yr-old brother; intestinal metaplasia (IIII) in the 47-yr-old brother, and a duodenal ulcer scar in the 42-yr-old sister. REP-PCR DNA fingerprints of H. pylori isolates from the index patient, his mother, and both of his brothers were identical or highly similar. By contrast, the H. pylori DNA fingerprint from the sister was markedly different from the H. pylori DNA fingerprints from the other family members. All isolates had the genotype cagA-positive and vacA slb/ml mosaic genotype. Conclusions: The DNA fingerprints of H. pylori strains obtained from members of this family with malignancy or premalignant histological disease were identical or highly similar and markedly different from the H. pylori DNA fingerprint from the sibling with duodenal ulcer disease. All H. pylori isolates within the family possessed genetic markers of enhanced virulence (presence of the cagA gene and vacA sl/ml mosaicism). In addition to host genetics and environmental factors, these findings suggest that infection with genetically similar H. pylori strains is a significant factor in determining the clinical outcome of an infection with H. pylori.

Original languageEnglish (US)
Pages (from-to)364-368
Number of pages5
JournalAmerican Journal of Gastroenterology
Volume94
Issue number2
DOIs
StatePublished - Feb 1999

Fingerprint

Helicobacter pylori
DNA Fingerprinting
Siblings
Genotype
Metaplasia
Duodenal Ulcer
Polymerase Chain Reaction
Stomach
Mothers
Duodenal Diseases
Marginal Zone B-Cell Lymphoma
Mosaicism
Gastrointestinal Diseases
Infection
Genetic Markers
Cicatrix
Virulence
Adenocarcinoma
DNA
Genes

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Molecular relationships of Helicobacter pylori strains in a family with gastroduodenal disease. / Miehlke, Stephan; Genta, Robert M.; Graham, David Y.; Go, Mae F.

In: American Journal of Gastroenterology, Vol. 94, No. 2, 02.1999, p. 364-368.

Research output: Contribution to journalArticle

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abstract = "Objective: Few studies have examined the genetic relationships of Helicobacter pylori strains affecting family members. Our aim was to do so. Methods: We characterized H. pylori isolates obtained from members of a single family presenting with various gastroduodenal diseases to examine H. pylori bacterial genetic similarity. Endoscopic evaluation with gastric mapping was performed on each individual to establish clinical and histological disease. Genomic DNA extracted from each H. pylori isolate was used to generate DNA fingerprints for each strain by REP-PCR. vacA genotypes and cagA presence were established by PCR. Results: Gastrointestinal diseases among the five members of this family included gastric adenocarcinoma in a 52-yr-old man (index patient), gastric MALT-lymphoma in the 73-yr-old mother; intestinal metaplasia (IV) and atrophic gastrifis in the 48-yr-old brother; intestinal metaplasia (IIII) in the 47-yr-old brother, and a duodenal ulcer scar in the 42-yr-old sister. REP-PCR DNA fingerprints of H. pylori isolates from the index patient, his mother, and both of his brothers were identical or highly similar. By contrast, the H. pylori DNA fingerprint from the sister was markedly different from the H. pylori DNA fingerprints from the other family members. All isolates had the genotype cagA-positive and vacA slb/ml mosaic genotype. Conclusions: The DNA fingerprints of H. pylori strains obtained from members of this family with malignancy or premalignant histological disease were identical or highly similar and markedly different from the H. pylori DNA fingerprint from the sibling with duodenal ulcer disease. All H. pylori isolates within the family possessed genetic markers of enhanced virulence (presence of the cagA gene and vacA sl/ml mosaicism). In addition to host genetics and environmental factors, these findings suggest that infection with genetically similar H. pylori strains is a significant factor in determining the clinical outcome of an infection with H. pylori.",
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