Molecular responses of vascular smooth muscle cells and phagocytes to curcumin-eluting bioresorbable stent materials

Kytai T. Nguyen, Nishat Shaikh, Kajal P. Shukla, Shih Horng Su, Robert C. Eberhart, Liping Tang

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

A major complication of coronary stenting is restenosis, often accompanied by inflammatory reactions and smooth muscle cell proliferation. Curcumin has been shown to possess anti-inflammatory and anti-proliferative properties, thus we hypothesize that locally released curcumin by coronary stent would diminish in-stent restenosis. As a first test of this hypothesis, curcumin-eluting PLLA films (C-PLLA) were produced and the anti-inflammatory and anti-proliferative properties were then tested using peritoneal phagocytes and human coronary artery smooth muscle cell (hCASMCs) culture systems. We find that the addition of curcumin reduced phagocyte accumulation and activation on C-PLLA films. On the other hand, C-PLLA significantly reduced the proliferation, but not the adhesion, of hCASMCs. The molecular responses of hCASMCs to C-PLLA were further assessed by cDNA microarray analysis. Curcumin up-regulated genes related to apoptosis and enhanced the expression of anti-proliferative and anti-inflammatory factors, and of antioxidants. Equally important, C-PLLA inhibited the cell cycle progression of adherent hCASMCs. The results suggest that curcumin regulates gene expression and cell function through the protein kinase (PK) and mitogen-activated protein kinase (MAPK) pathways. These results support the use of curcumin to inhibit in-stent restenosis.

Original languageEnglish (US)
Pages (from-to)5333-5346
Number of pages14
JournalBiomaterials
Volume25
Issue number23
DOIs
StatePublished - Oct 1 2004

Keywords

  • Cell proliferation
  • Drug delivery
  • Gene expression
  • Inflammation
  • Phagocytes
  • Polylactic acid
  • Restenosis
  • Smooth muscle cells

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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