Molecular structure of cytoplasmic dynein 2 and its distribution in neuronal and ciliated cells

Atsushi Mikami, Sharon H. Tynan, Taro Hama, Katherine Luby-Phelps, Tetsuichiro Saito, James E. Crandall, Joseph C. Besharse, Richard B. Valle

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Cytoplasmic dynein is involved in a wide variety of cellular functions. In addition to the initially characterized form (MAP 1C/dynein 1), a second form of cytoplasmic dynein (dynein 2) has been identified and implicated in intraflagellar transport (IFT) in lower eukaryotes and in Golgi organization in vertebrates. In the current study, the primary structure of the full-length dynein 2 heavy chain (HC) was determined from cDNA sequence. The dynein 1 and dynein 2 sequences were similar within the motor region, and around the light intermediate chain (LIC)-binding site within the N-terminal stem region. The dynein 2 HC co-immunoprecipitated with LIC3, a homologue of dynein 1 LICs. Dynein 2 mRNA was abundant in the ependymal layer of the neural tube and in the olfactory epithelium. Antibodies to dynein 2 HC, LIC3 and a component of IFT particles strongly stained the ependymal layer lining the lateral ventricles. Both dynein 2 HC and LIC3 staining was also observed associated with connecting cilia in the retina and within primary cilia of non-neuronal cultured cells. These data support a specific role for dynein 2 in the generation and maintenance of cilia.

Original languageEnglish (US)
Pages (from-to)4801-4808
Number of pages8
JournalJournal of Cell Science
Volume115
Issue number24
DOIs
StatePublished - Dec 15 2002

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Cytoplasmic Dyneins
Dyneins
Molecular Structure
Cilia
Olfactory Mucosa
Neural Tube
Lateral Ventricles
Eukaryota
Vertebrates
Retina
Cultured Cells

Keywords

  • Brain
  • Cilium
  • Dynein
  • Light intermediate cham
  • Microtubule
  • Retina

ASJC Scopus subject areas

  • Cell Biology

Cite this

Mikami, A., Tynan, S. H., Hama, T., Luby-Phelps, K., Saito, T., Crandall, J. E., ... Valle, R. B. (2002). Molecular structure of cytoplasmic dynein 2 and its distribution in neuronal and ciliated cells. Journal of Cell Science, 115(24), 4801-4808. https://doi.org/10.1242/jcs.00168

Molecular structure of cytoplasmic dynein 2 and its distribution in neuronal and ciliated cells. / Mikami, Atsushi; Tynan, Sharon H.; Hama, Taro; Luby-Phelps, Katherine; Saito, Tetsuichiro; Crandall, James E.; Besharse, Joseph C.; Valle, Richard B.

In: Journal of Cell Science, Vol. 115, No. 24, 15.12.2002, p. 4801-4808.

Research output: Contribution to journalArticle

Mikami, A, Tynan, SH, Hama, T, Luby-Phelps, K, Saito, T, Crandall, JE, Besharse, JC & Valle, RB 2002, 'Molecular structure of cytoplasmic dynein 2 and its distribution in neuronal and ciliated cells', Journal of Cell Science, vol. 115, no. 24, pp. 4801-4808. https://doi.org/10.1242/jcs.00168
Mikami, Atsushi ; Tynan, Sharon H. ; Hama, Taro ; Luby-Phelps, Katherine ; Saito, Tetsuichiro ; Crandall, James E. ; Besharse, Joseph C. ; Valle, Richard B. / Molecular structure of cytoplasmic dynein 2 and its distribution in neuronal and ciliated cells. In: Journal of Cell Science. 2002 ; Vol. 115, No. 24. pp. 4801-4808.
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