Molecular structure of double reciprocal translocations: Significance in B-cell lymphomagenesis

P. R K Koduru, K. Offit

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The molecular structure of reciprocal translocations associated with low grade and high grade non-Hodgkin's lymphomas occurring together was analysed in two tumors. Sequential biopsies documented histological transformation of a large cell lymphoma to an immunoblastic lymphoma bearing t(14;18)(q32;q21) and t(8;22)(q24;q11). A second tumor, a small non-cleaved cell lymphoma, demonstrated a t(8;14)(q24;q11) as well as t(18;22)(q21;q11). DNA analysis from these tumors showed rearrangements at the Ig heavy chain, kappa and lambda light chains, BCL2 and c-MYC loci. Utilizing multiple enzyme digests and different probes spanning the BCL2, c-MYC and Ig genes, mapping of DNA break-points was performed. In both these tumors primary translocation events dysregulating the BCL2 or c-MYC were identified to have occurred in a pre-B-cell. Based on these results and those published previously, a sequence of B-cell development during which somatic recombination errors lead to the genesis of specific translocations is proposed. From these studies it is inferred that secondary dysregulation of a c-MYC in a lymphoma tumor carrying dysregulated BCL2 gene leads to rapid progression to high grade disease.

Original languageEnglish (US)
Pages (from-to)145-148
Number of pages4
JournalOncogene
Volume6
Issue number1
StatePublished - Jan 1991

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Molecular Structure
B-Lymphocytes
Non-Hodgkin's Lymphoma
Neoplasms
Lymphoma
Large-Cell Immunoblastic Lymphoma
Immunoglobulin Heavy Chains
Immunoglobulin Genes
DNA Breaks
B-Lymphoid Precursor Cells
Chromosome Mapping
Genetic Recombination
Biopsy
Light
DNA
Enzymes
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Molecular structure of double reciprocal translocations : Significance in B-cell lymphomagenesis. / Koduru, P. R K; Offit, K.

In: Oncogene, Vol. 6, No. 1, 01.1991, p. 145-148.

Research output: Contribution to journalArticle

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