Molecular ultrasound imaging using a novel tumor-bearing animal model with variable target receptor expression

Anna G. Sorace, Reshu Saini, Marshall Mahoney, Jason M. Warram, Kurt R. Zinn, Kenneth Hoyt

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Molecular ultrasound (US) imaging of overexpressed endothelial receptors has been shown to increase tumor visualization within a region-of-interest and is of particular interest in cancer research. This study used an in vivo animal model system for evaluating targeted microbubble (MB) contrast agents. 2LMP breast cancer-bearing mice (N = 6) expressed varying intratumoral levels of the target hemagglutinin (HA) receptor through adenoviral (Ad) vector modulation. Animals were administered a 'low' and then a 'high' Ad vector dose following initial US imaging. Anti-HA antibody-labeled or control MBs were prepared and molecular US imaging was performed 24 hours after Ad vector infection using a SONIX Tablet ultrasound system (Ultrasonix Medical Corp). Custom Matlab programs were developed to evaluate US image intensity from data collected using a MB destruction-replenishment technique. Results showed intratumoral enhancement due to targeted MB accumulation was significantly increased in the 'high' Ad vector dosed animals compared to the same animals administered a 'low' Ad vector dose (P = 0.001). Control MBs showed no significant differences between imaging days (P = 0.96). Additionally, targeted MBs on average produced a 10.5-fold increase in intratumoral image intensity for the 'low' HA receptor group and an 18.8-fold increase in image intensity for the 'high' HA receptor group compared to control MBs.

Original languageEnglish (US)
Title of host publication2012 IEEE International Ultrasonics Symposium, IUS 2012
Pages2049-2051
Number of pages3
DOIs
StatePublished - Dec 1 2012
Event2012 IEEE International Ultrasonics Symposium, IUS 2012 - Dresden, Germany
Duration: Oct 7 2012Oct 10 2012

Publication series

NameIEEE International Ultrasonics Symposium, IUS
ISSN (Print)1948-5719
ISSN (Electronic)1948-5727

Other

Other2012 IEEE International Ultrasonics Symposium, IUS 2012
CountryGermany
CityDresden
Period10/7/1210/10/12

ASJC Scopus subject areas

  • Acoustics and Ultrasonics

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    Sorace, A. G., Saini, R., Mahoney, M., Warram, J. M., Zinn, K. R., & Hoyt, K. (2012). Molecular ultrasound imaging using a novel tumor-bearing animal model with variable target receptor expression. In 2012 IEEE International Ultrasonics Symposium, IUS 2012 (pp. 2049-2051). [6562192] (IEEE International Ultrasonics Symposium, IUS). https://doi.org/10.1109/ULTSYM.2012.0512