Monitoring drug induced apoptosis and treatment sensitivity in non-small cell lung carcinoma using dielectrophoresis

Rajeshwari Taruvai Kalyana Kumar, Shanshan Liu, John D. Minna, Shalini Prasad

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Non-invasive real time methods for characterizing biomolecular events that contribute towards apoptotic kinetics would be of significant importance in the field of cancer biology. Effective drug-induced apoptosis is an important factor for establishing the relationship between cancer genetics and treatment sensitivity. The objective of this study was to develop a non-invasive technique to characterize cancer cells that are undergoing drug-induced apoptosis. We used dielectrophoresis to determine apoptotic cells as early as 2 h post drug treatment as compared to 24 h with standard flow cytometry method using non-small cell lung cancer (NSCLC) adenocarcinoma cell line (HCC1833) as a study model. Our studies have shown significant differences in apoptotic cells by chromatin condensation, formation of apoptotic bodies and exposure of phosphatidylserine (PS) on the extracellular surface when the cells where treated with a potent Bcl-2 family inhibitor drug (ABT-263). Time lapse dielectrophoretic studies were performed over 24 h period after exposure to ABT-263 at clinically relevant concentrations. The dielectrophoretic studies were compared to Annexin-V FITC flow assay for the detection of PS in mid-stage apoptosis using flow cytometry. As a result of physical and biochemical changes, inherent dielectric properties of cells undergoing varying stages of apoptosis showed amplified changes in their cytoplasmic and membrane capacitance. In addition, zeta potential of these fixed isolated cells was measured to obtain direct correlation to biomolecular events.

Original languageEnglish (US)
Pages (from-to)1877-1883
Number of pages7
JournalBiochimica et Biophysica Acta - General Subjects
Volume1860
Issue number9
DOIs
StatePublished - Sep 1 2016

Fingerprint

Drug Monitoring
Electrophoresis
Non-Small Cell Lung Carcinoma
Cells
Apoptosis
Monitoring
Pharmaceutical Preparations
Flow cytometry
Phosphatidylserines
Flow Cytometry
Drug therapy
Fluorescein-5-isothiocyanate
Annexin A5
Zeta potential
Neoplasms
Dielectric properties
Chromatin
Condensation
Assays
Capacitance

Keywords

  • Apoptosis monitoring
  • Dielectrophoresis
  • Non invasive electrokinetics
  • Non-small cell lung cancer

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Monitoring drug induced apoptosis and treatment sensitivity in non-small cell lung carcinoma using dielectrophoresis. / Taruvai Kalyana Kumar, Rajeshwari; Liu, Shanshan; Minna, John D.; Prasad, Shalini.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1860, No. 9, 01.09.2016, p. 1877-1883.

Research output: Contribution to journalArticle

@article{8d08fac63f254c619946b0f3352f5b43,
title = "Monitoring drug induced apoptosis and treatment sensitivity in non-small cell lung carcinoma using dielectrophoresis",
abstract = "Non-invasive real time methods for characterizing biomolecular events that contribute towards apoptotic kinetics would be of significant importance in the field of cancer biology. Effective drug-induced apoptosis is an important factor for establishing the relationship between cancer genetics and treatment sensitivity. The objective of this study was to develop a non-invasive technique to characterize cancer cells that are undergoing drug-induced apoptosis. We used dielectrophoresis to determine apoptotic cells as early as 2 h post drug treatment as compared to 24 h with standard flow cytometry method using non-small cell lung cancer (NSCLC) adenocarcinoma cell line (HCC1833) as a study model. Our studies have shown significant differences in apoptotic cells by chromatin condensation, formation of apoptotic bodies and exposure of phosphatidylserine (PS) on the extracellular surface when the cells where treated with a potent Bcl-2 family inhibitor drug (ABT-263). Time lapse dielectrophoretic studies were performed over 24 h period after exposure to ABT-263 at clinically relevant concentrations. The dielectrophoretic studies were compared to Annexin-V FITC flow assay for the detection of PS in mid-stage apoptosis using flow cytometry. As a result of physical and biochemical changes, inherent dielectric properties of cells undergoing varying stages of apoptosis showed amplified changes in their cytoplasmic and membrane capacitance. In addition, zeta potential of these fixed isolated cells was measured to obtain direct correlation to biomolecular events.",
keywords = "Apoptosis monitoring, Dielectrophoresis, Non invasive electrokinetics, Non-small cell lung cancer",
author = "{Taruvai Kalyana Kumar}, Rajeshwari and Shanshan Liu and Minna, {John D.} and Shalini Prasad",
year = "2016",
month = "9",
day = "1",
doi = "10.1016/j.bbagen.2016.05.039",
language = "English (US)",
volume = "1860",
pages = "1877--1883",
journal = "Biochimica et Biophysica Acta - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "9",

}

TY - JOUR

T1 - Monitoring drug induced apoptosis and treatment sensitivity in non-small cell lung carcinoma using dielectrophoresis

AU - Taruvai Kalyana Kumar, Rajeshwari

AU - Liu, Shanshan

AU - Minna, John D.

AU - Prasad, Shalini

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Non-invasive real time methods for characterizing biomolecular events that contribute towards apoptotic kinetics would be of significant importance in the field of cancer biology. Effective drug-induced apoptosis is an important factor for establishing the relationship between cancer genetics and treatment sensitivity. The objective of this study was to develop a non-invasive technique to characterize cancer cells that are undergoing drug-induced apoptosis. We used dielectrophoresis to determine apoptotic cells as early as 2 h post drug treatment as compared to 24 h with standard flow cytometry method using non-small cell lung cancer (NSCLC) adenocarcinoma cell line (HCC1833) as a study model. Our studies have shown significant differences in apoptotic cells by chromatin condensation, formation of apoptotic bodies and exposure of phosphatidylserine (PS) on the extracellular surface when the cells where treated with a potent Bcl-2 family inhibitor drug (ABT-263). Time lapse dielectrophoretic studies were performed over 24 h period after exposure to ABT-263 at clinically relevant concentrations. The dielectrophoretic studies were compared to Annexin-V FITC flow assay for the detection of PS in mid-stage apoptosis using flow cytometry. As a result of physical and biochemical changes, inherent dielectric properties of cells undergoing varying stages of apoptosis showed amplified changes in their cytoplasmic and membrane capacitance. In addition, zeta potential of these fixed isolated cells was measured to obtain direct correlation to biomolecular events.

AB - Non-invasive real time methods for characterizing biomolecular events that contribute towards apoptotic kinetics would be of significant importance in the field of cancer biology. Effective drug-induced apoptosis is an important factor for establishing the relationship between cancer genetics and treatment sensitivity. The objective of this study was to develop a non-invasive technique to characterize cancer cells that are undergoing drug-induced apoptosis. We used dielectrophoresis to determine apoptotic cells as early as 2 h post drug treatment as compared to 24 h with standard flow cytometry method using non-small cell lung cancer (NSCLC) adenocarcinoma cell line (HCC1833) as a study model. Our studies have shown significant differences in apoptotic cells by chromatin condensation, formation of apoptotic bodies and exposure of phosphatidylserine (PS) on the extracellular surface when the cells where treated with a potent Bcl-2 family inhibitor drug (ABT-263). Time lapse dielectrophoretic studies were performed over 24 h period after exposure to ABT-263 at clinically relevant concentrations. The dielectrophoretic studies were compared to Annexin-V FITC flow assay for the detection of PS in mid-stage apoptosis using flow cytometry. As a result of physical and biochemical changes, inherent dielectric properties of cells undergoing varying stages of apoptosis showed amplified changes in their cytoplasmic and membrane capacitance. In addition, zeta potential of these fixed isolated cells was measured to obtain direct correlation to biomolecular events.

KW - Apoptosis monitoring

KW - Dielectrophoresis

KW - Non invasive electrokinetics

KW - Non-small cell lung cancer

UR - http://www.scopus.com/inward/record.url?scp=84974539363&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84974539363&partnerID=8YFLogxK

U2 - 10.1016/j.bbagen.2016.05.039

DO - 10.1016/j.bbagen.2016.05.039

M3 - Article

C2 - 27262539

AN - SCOPUS:84974539363

VL - 1860

SP - 1877

EP - 1883

JO - Biochimica et Biophysica Acta - General Subjects

JF - Biochimica et Biophysica Acta - General Subjects

SN - 0304-4165

IS - 9

ER -