Monitoring of microbubble-mediated ultrasound therapy using fluorescent imaging: A feasibility study

A. G. Sorace, R. Saini, E. Rosenthal, K. Hoyt

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Microbubble-mediated ultrasound therapy has been shown to noninvasively increase drug uptake through increasing cell membrane permeability and vascular extravasation. This technique has potential to improve drug uptake in localized areas of cancer. Using optical imaging to monitor fluorescent tracer uptake is a non-invasive method for increasing information on microbubble-mediated ultrasound therapy. Mice (N = 10) were implanted with 2LMP breast cancer cells and injected with Cy5.5 fluorescent dye before undergoing microbubble-mediated ultrasound therapy. Post therapy, animals were transiently imaged at 1, 10, 30 and 60 min using a small animal optical imaging system. Tumors were extracted and analyzed for fluorescent signal. Using ROI analysis, the microbubble-mediated ultrasound therapy group showed increased uptake compared to control group (p = 0.02). Supernatant from tumors, post extraction, also showed an increased fluorescent dye compared to control counterparts (p = 0.01). In vivo fluorescent imaging of microbubble-mediated ultrasound therapy allows monitoring of uptake in a localized area.

Original languageEnglish (US)
Title of host publication2011 IEEE International Ultrasonics Symposium, IUS 2011
Pages1902-1905
Number of pages4
DOIs
StatePublished - Dec 1 2011
Event2011 IEEE International Ultrasonics Symposium, IUS 2011 - Orlando, FL, United States
Duration: Oct 18 2011Oct 21 2011

Publication series

NameIEEE International Ultrasonics Symposium, IUS
ISSN (Print)1948-5719
ISSN (Electronic)1948-5727

Other

Other2011 IEEE International Ultrasonics Symposium, IUS 2011
CountryUnited States
CityOrlando, FL
Period10/18/1110/21/11

ASJC Scopus subject areas

  • Acoustics and Ultrasonics

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    Sorace, A. G., Saini, R., Rosenthal, E., & Hoyt, K. (2011). Monitoring of microbubble-mediated ultrasound therapy using fluorescent imaging: A feasibility study. In 2011 IEEE International Ultrasonics Symposium, IUS 2011 (pp. 1902-1905). [6293581] (IEEE International Ultrasonics Symposium, IUS). https://doi.org/10.1109/ULTSYM.2011.0475