Monitoring of thermal dose during ablation therapy using quantum dot-mediated fluorescence thermometry

Karim Bensalah, Altug Tuncel, Willard Hanson, Joshua Stern, Bumsoo Han, Jeffrey A Cadeddu

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Purpose: The objective of this study was to demonstrate the feasibility of quantum dot (QD)-mediated fluorescence thermometry to monitor thermal dose in an in-vitro thermal ablation zone generated by laser-heated gold nanoshells (LGNS). Materials and Methods: Hyperthermic cell death of human prostate cancer cell line (PC-3) was determined after various heating settings and correlated to the thermal conditions using an Arrhenius model prior to LGNS ablation. PC-3cells with gold nanoshells (GNS) and QDs were exposed to a near-infrared laser and QD excitation light. When the cells were heated by GNS, local temperature was measured using the temperature-dependent fluorescence intensity of QDs. Using the predetermined Arrhenius model, the thermal dose (i.e., cell death of PC-3cells) by LGNS was estimated with local temperatures measured with QD-mediated thermometry. The estimated thermal dose was confirmed with calcein-acetoxy-methylester viability assay. Results: For PC-3cell line, the activation energy and frequency factor of the Arrhenius model were 86.78kcal/mol and 6.35×1055 Hz, respectively. During LGNS ablation of PC-3cells, QD-mediated temperature measurement showed that the temperature of the laser spot increased rapidly to ∼58°C±4°C. The estimated thermal dose showed that cell death reached to ∼90% in 120 seconds. The death cell zone observed after staining corresponded to a peak area of the temperature profile generated after analysis of the QD fluorescence intensity. Conclusions: This study shows that the QD fluorescence thermometry can accurately monitor the PC-3cell death by LGNS ablation. This approach holds promises for a better monitoring of thermal ablation procedures in clinical practice.

Original languageEnglish (US)
Pages (from-to)1903-1908
Number of pages6
JournalJournal of Endourology
Volume24
Issue number12
DOIs
StatePublished - Dec 1 2010

Fingerprint

Nanoshells
Thermometry
Quantum Dots
Gold
Lasers
Hot Temperature
Fluorescence
Temperature
Cell Death
Therapeutics
Heating
Prostatic Neoplasms
Staining and Labeling
Light
Cell Line

ASJC Scopus subject areas

  • Urology

Cite this

Monitoring of thermal dose during ablation therapy using quantum dot-mediated fluorescence thermometry. / Bensalah, Karim; Tuncel, Altug; Hanson, Willard; Stern, Joshua; Han, Bumsoo; Cadeddu, Jeffrey A.

In: Journal of Endourology, Vol. 24, No. 12, 01.12.2010, p. 1903-1908.

Research output: Contribution to journalArticle

Bensalah, Karim ; Tuncel, Altug ; Hanson, Willard ; Stern, Joshua ; Han, Bumsoo ; Cadeddu, Jeffrey A. / Monitoring of thermal dose during ablation therapy using quantum dot-mediated fluorescence thermometry. In: Journal of Endourology. 2010 ; Vol. 24, No. 12. pp. 1903-1908.
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abstract = "Purpose: The objective of this study was to demonstrate the feasibility of quantum dot (QD)-mediated fluorescence thermometry to monitor thermal dose in an in-vitro thermal ablation zone generated by laser-heated gold nanoshells (LGNS). Materials and Methods: Hyperthermic cell death of human prostate cancer cell line (PC-3) was determined after various heating settings and correlated to the thermal conditions using an Arrhenius model prior to LGNS ablation. PC-3cells with gold nanoshells (GNS) and QDs were exposed to a near-infrared laser and QD excitation light. When the cells were heated by GNS, local temperature was measured using the temperature-dependent fluorescence intensity of QDs. Using the predetermined Arrhenius model, the thermal dose (i.e., cell death of PC-3cells) by LGNS was estimated with local temperatures measured with QD-mediated thermometry. The estimated thermal dose was confirmed with calcein-acetoxy-methylester viability assay. Results: For PC-3cell line, the activation energy and frequency factor of the Arrhenius model were 86.78kcal/mol and 6.35×1055 Hz, respectively. During LGNS ablation of PC-3cells, QD-mediated temperature measurement showed that the temperature of the laser spot increased rapidly to ∼58°C±4°C. The estimated thermal dose showed that cell death reached to ∼90{\%} in 120 seconds. The death cell zone observed after staining corresponded to a peak area of the temperature profile generated after analysis of the QD fluorescence intensity. Conclusions: This study shows that the QD fluorescence thermometry can accurately monitor the PC-3cell death by LGNS ablation. This approach holds promises for a better monitoring of thermal ablation procedures in clinical practice.",
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