Monoclonal antibodies directed against a cell surface exposed outer membrane protein of Haemophilus influenzae type b

S. M. Robertson, C. F. Frisch, P. A. Gulig, J. R. Kettman, K. H. Johnston, E. J. Hansen

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Monoclonal antibodies directed against several different Haemophilus influenzae type b outer membrane proteins with apparent molecular weights of 45,000, 39,000, and 37,000 were identified by a radioimmunoprecipitation method. Five monoclonal antibodies, including both immunoglobulin G and M isotypes, were specific for the same H. influenzae type b major outer membrane protein (39,000 molecular weight). One of these immunoglobulin G monoclonal antibodies (6A2) was shown to be directed against a cell surface-exposed antigenic determinant of the 39,000-molecular-weight protein, whereas the other monoclonal antibodies directed against this same protein were apparently specific for antigenic determinants not exposed on the H. influenzae type b cell surface. The cell surface-exposed protein antigenic determinant recognized by monoclonal antibody 6A2 was not unique to H. influenzae type b strain used as the source of outer membrane vesicles for generating immune spleen cells, but was found in a majority of independently isolated strains of H. influenzae type b. These data indicate that there is antigenic cross-reactivity among H. influenzae type b strains with regard to cell surface-exposed proteins.

Original languageEnglish (US)
Pages (from-to)80-88
Number of pages9
JournalUnknown Journal
Volume36
Issue number1
DOIs
StatePublished - 1982

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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