Monoclonal antibodies directed against a cell surface exposed outer membrane protein of Haemophilus influenzae type b

S. M. Robertson, C. F. Frisch, P. A. Gulig, J. R. Kettman, K. H. Johnston, E. J. Hansen

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Abstract

Monoclonal antibodies directed against several different Haemophilus influenzae type b outer membrane proteins with apparent molecular weights of 45,000, 39,000, and 37,000 were identified by a radioimmunoprecipitation method. Five monoclonal antibodies, including both immunoglobulin G and M isotypes, were specific for the same H. influenzae type b major outer membrane protein (39,000 molecular weight). One of these immunoglobulin G monoclonal antibodies (6A2) was shown to be directed against a cell surface-exposed antigenic determinant of the 39,000-molecular-weight protein, whereas the other monoclonal antibodies directed against this same protein were apparently specific for antigenic determinants not exposed on the H. influenzae type b cell surface. The cell surface-exposed protein antigenic determinant recognized by monoclonal antibody 6A2 was not unique to H. influenzae type b strain used as the source of outer membrane vesicles for generating immune spleen cells, but was found in a majority of independently isolated strains of H. influenzae type b. These data indicate that there is antigenic cross-reactivity among H. influenzae type b strains with regard to cell surface-exposed proteins.

Original languageEnglish (US)
Pages (from-to)80-88
Number of pages9
JournalInfection and Immunity
Volume36
Issue number1
StatePublished - 1982

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Haemophilus influenzae type b
Membrane Proteins
Monoclonal Antibodies
Epitopes
Molecular Weight
Immunoglobulin G
Immunoglobulin M
Proteins
Spleen
Membranes

ASJC Scopus subject areas

  • Immunology

Cite this

Robertson, S. M., Frisch, C. F., Gulig, P. A., Kettman, J. R., Johnston, K. H., & Hansen, E. J. (1982). Monoclonal antibodies directed against a cell surface exposed outer membrane protein of Haemophilus influenzae type b. Infection and Immunity, 36(1), 80-88.

Monoclonal antibodies directed against a cell surface exposed outer membrane protein of Haemophilus influenzae type b. / Robertson, S. M.; Frisch, C. F.; Gulig, P. A.; Kettman, J. R.; Johnston, K. H.; Hansen, E. J.

In: Infection and Immunity, Vol. 36, No. 1, 1982, p. 80-88.

Research output: Contribution to journalArticle

Robertson, SM, Frisch, CF, Gulig, PA, Kettman, JR, Johnston, KH & Hansen, EJ 1982, 'Monoclonal antibodies directed against a cell surface exposed outer membrane protein of Haemophilus influenzae type b', Infection and Immunity, vol. 36, no. 1, pp. 80-88.
Robertson, S. M. ; Frisch, C. F. ; Gulig, P. A. ; Kettman, J. R. ; Johnston, K. H. ; Hansen, E. J. / Monoclonal antibodies directed against a cell surface exposed outer membrane protein of Haemophilus influenzae type b. In: Infection and Immunity. 1982 ; Vol. 36, No. 1. pp. 80-88.
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AU - Frisch, C. F.

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AU - Johnston, K. H.

AU - Hansen, E. J.

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