Monoclonal Antibodies to a Human Prostate Antigen

A. E. Frankel, R. V. Rouse, M. C. Wang, T. M. Chu, L. A. Herzenberg

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Three monoclonal antibodies reactive with a purified extractable Mr 34,000 prostate antigen (PA) have been prepared by fusing splenocytes of BALB/c mice preimmunized with purified PA with the NS1 mouse myeloma cell line. The three antibodies were all of the lgG-1 subclass. The antibodies defined two non-cross-blocking unique determinants on PA; each present as one site per molecule. IF3 defined one antigenic site and 2G7 and 1C5 defined another antigenic determinant. All of the antibodies reacted with PA in a solid-phase radioimmunoassay and immunoprecipitated 125I-labeled PA. Absorption and sandwich radioimmunoassays showed PA in prostate tissues but not in tonsil, liver, or kidney. Immunoperoxidase staining of formalin-fixed paraffin-embedded sections of benign prostatic hyperplasia and prostatic carcinoma revealed strong prostate epithelial reactivity. None of the antibodies showed reactivity with prostate membrane preparations. A sandwich radioimmunoassay used 2G7 as a plate coat. 125I-labeled 1F3 was used to detect 5 ng PA per ml in sera of patients with prostate cancer. These results confirm previous observations regarding the specificity of PA and shed new evidence for its intracellular localization.

Original languageEnglish (US)
Pages (from-to)3714-3718
Number of pages5
JournalCancer research
Volume42
Issue number9
StatePublished - Sep 1 1982

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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