Abstract
We established 16 monoclonal antibodies (MAbs) to endothelin (ET), a novel and potent vasoconstrictor. They were classified into two groups: eight of them reacted with the C-terminal portion of ET, and the other eight reacted with the non-C-terminal portion. We constructed the sandwich enzyme linked immunosorbent assays (ELISAs) using a combination of a MAb belonging to the former with that belonging to the latter, and could detect about 400 pg/ml of ET.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 63-69 |
| Number of pages | 7 |
| Journal | Hybridoma |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1990 |
ASJC Scopus subject areas
- Immunology
- Genetics
Fingerprint Dive into the research topics of 'Monoclonal antibodies to endothelin: Application for sandwich immunoassays'. Together they form a unique fingerprint.
Cite this
Hamaoki, M., Kato, H., Sugi, M., Fujimoto, M., Kurihara, H., Yosizumi, M., Yanagisawa, M., Kimura, S., Masaki, T., & Yazaki, Y. (1990). Monoclonal antibodies to endothelin: Application for sandwich immunoassays. Hybridoma, 9(1), 63-69. https://doi.org/10.1089/hyb.1990.9.63