Abstract
We established 16 monoclonal antibodies (MAbs) to endothelin (ET), a novel and potent vasoconstrictor. They were classified into two groups: eight of them reacted with the C-terminal portion of ET, and the other eight reacted with the non-C-terminal portion. We constructed the sandwich enzyme linked immunosorbent assays (ELISAs) using a combination of a MAb belonging to the former with that belonging to the latter, and could detect about 400 pg/ml of ET.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 63-69 |
| Number of pages | 7 |
| Journal | Hybridoma |
| Volume | 9 |
| Issue number | 1 |
| State | Published - 1990 |
Fingerprint
ASJC Scopus subject areas
- Immunology
- Genetics
Cite this
Monoclonal antibodies to endothelin : Application for sandwich immunoassays. / Hamaoki, M.; Kato, H.; Sugi, M.; Fujimoto, M.; Kurihara, H.; Yosizumi, M.; Yanagisawa, M.; Kimura, S.; Masaki, T.; Yazaki, Y.
In: Hybridoma, Vol. 9, No. 1, 1990, p. 63-69.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Monoclonal antibodies to endothelin
T2 - Application for sandwich immunoassays
AU - Hamaoki, M.
AU - Kato, H.
AU - Sugi, M.
AU - Fujimoto, M.
AU - Kurihara, H.
AU - Yosizumi, M.
AU - Yanagisawa, M.
AU - Kimura, S.
AU - Masaki, T.
AU - Yazaki, Y.
PY - 1990
Y1 - 1990
N2 - We established 16 monoclonal antibodies (MAbs) to endothelin (ET), a novel and potent vasoconstrictor. They were classified into two groups: eight of them reacted with the C-terminal portion of ET, and the other eight reacted with the non-C-terminal portion. We constructed the sandwich enzyme linked immunosorbent assays (ELISAs) using a combination of a MAb belonging to the former with that belonging to the latter, and could detect about 400 pg/ml of ET.
AB - We established 16 monoclonal antibodies (MAbs) to endothelin (ET), a novel and potent vasoconstrictor. They were classified into two groups: eight of them reacted with the C-terminal portion of ET, and the other eight reacted with the non-C-terminal portion. We constructed the sandwich enzyme linked immunosorbent assays (ELISAs) using a combination of a MAb belonging to the former with that belonging to the latter, and could detect about 400 pg/ml of ET.
UR - http://www.scopus.com/inward/record.url?scp=0025210101&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025210101&partnerID=8YFLogxK
M3 - Article
C2 - 2179120
AN - SCOPUS:0025210101
VL - 9
SP - 63
EP - 69
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
SN - 2167-9436
IS - 1
ER -