TY - JOUR
T1 - Monoclonal antibodies to endothelin
T2 - Application for sandwich immunoassays
AU - Hamaoki, M.
AU - Kato, H.
AU - Sugi, M.
AU - Fujimoto, M.
AU - Kurihara, H.
AU - Yosizumi, M.
AU - Yanagisawa, M.
AU - Kimura, S.
AU - Masaki, T.
AU - Yazaki, Y.
PY - 1990
Y1 - 1990
N2 - We established 16 monoclonal antibodies (MAbs) to endothelin (ET), a novel and potent vasoconstrictor. They were classified into two groups: eight of them reacted with the C-terminal portion of ET, and the other eight reacted with the non-C-terminal portion. We constructed the sandwich enzyme linked immunosorbent assays (ELISAs) using a combination of a MAb belonging to the former with that belonging to the latter, and could detect about 400 pg/ml of ET.
AB - We established 16 monoclonal antibodies (MAbs) to endothelin (ET), a novel and potent vasoconstrictor. They were classified into two groups: eight of them reacted with the C-terminal portion of ET, and the other eight reacted with the non-C-terminal portion. We constructed the sandwich enzyme linked immunosorbent assays (ELISAs) using a combination of a MAb belonging to the former with that belonging to the latter, and could detect about 400 pg/ml of ET.
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U2 - 10.1089/hyb.1990.9.63
DO - 10.1089/hyb.1990.9.63
M3 - Article
C2 - 2179120
AN - SCOPUS:0025210101
VL - 9
SP - 63
EP - 69
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
SN - 2167-9436
IS - 1
ER -