Monoclonal antibodies to endothelin: Application for sandwich immunoassays

M. Hamaoki, H. Kato, M. Sugi, M. Fujimoto, H. Kurihara, M. Yosizumi, M. Yanagisawa, S. Kimura, T. Masaki, Y. Yazaki

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We established 16 monoclonal antibodies (MAbs) to endothelin (ET), a novel and potent vasoconstrictor. They were classified into two groups: eight of them reacted with the C-terminal portion of ET, and the other eight reacted with the non-C-terminal portion. We constructed the sandwich enzyme linked immunosorbent assays (ELISAs) using a combination of a MAb belonging to the former with that belonging to the latter, and could detect about 400 pg/ml of ET.

Original languageEnglish (US)
Pages (from-to)63-69
Number of pages7
JournalHybridoma
Volume9
Issue number1
StatePublished - 1990

Fingerprint

Endothelins
Immunoassay
Monoclonal Antibodies
Vasoconstrictor Agents
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Hamaoki, M., Kato, H., Sugi, M., Fujimoto, M., Kurihara, H., Yosizumi, M., ... Yazaki, Y. (1990). Monoclonal antibodies to endothelin: Application for sandwich immunoassays. Hybridoma, 9(1), 63-69.

Monoclonal antibodies to endothelin : Application for sandwich immunoassays. / Hamaoki, M.; Kato, H.; Sugi, M.; Fujimoto, M.; Kurihara, H.; Yosizumi, M.; Yanagisawa, M.; Kimura, S.; Masaki, T.; Yazaki, Y.

In: Hybridoma, Vol. 9, No. 1, 1990, p. 63-69.

Research output: Contribution to journalArticle

Hamaoki, M, Kato, H, Sugi, M, Fujimoto, M, Kurihara, H, Yosizumi, M, Yanagisawa, M, Kimura, S, Masaki, T & Yazaki, Y 1990, 'Monoclonal antibodies to endothelin: Application for sandwich immunoassays', Hybridoma, vol. 9, no. 1, pp. 63-69.
Hamaoki M, Kato H, Sugi M, Fujimoto M, Kurihara H, Yosizumi M et al. Monoclonal antibodies to endothelin: Application for sandwich immunoassays. Hybridoma. 1990;9(1):63-69.
Hamaoki, M. ; Kato, H. ; Sugi, M. ; Fujimoto, M. ; Kurihara, H. ; Yosizumi, M. ; Yanagisawa, M. ; Kimura, S. ; Masaki, T. ; Yazaki, Y. / Monoclonal antibodies to endothelin : Application for sandwich immunoassays. In: Hybridoma. 1990 ; Vol. 9, No. 1. pp. 63-69.
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