Monoclonal antibodies to endothelin: Application for sandwich immunoassays

M. Hamaoki, H. Kato, M. Sugi, M. Fujimoto, H. Kurihara, M. Yosizumi, M. Yanagisawa, S. Kimura, T. Masaki, Y. Yazaki

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

We established 16 monoclonal antibodies (MAbs) to endothelin (ET), a novel and potent vasoconstrictor. They were classified into two groups: eight of them reacted with the C-terminal portion of ET, and the other eight reacted with the non-C-terminal portion. We constructed the sandwich enzyme linked immunosorbent assays (ELISAs) using a combination of a MAb belonging to the former with that belonging to the latter, and could detect about 400 pg/ml of ET.

Original languageEnglish (US)
Pages (from-to)63-69
Number of pages7
JournalHybridoma
Volume9
Issue number1
DOIs
StatePublished - 1990

ASJC Scopus subject areas

  • Immunology
  • Genetics

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    Hamaoki, M., Kato, H., Sugi, M., Fujimoto, M., Kurihara, H., Yosizumi, M., Yanagisawa, M., Kimura, S., Masaki, T., & Yazaki, Y. (1990). Monoclonal antibodies to endothelin: Application for sandwich immunoassays. Hybridoma, 9(1), 63-69. https://doi.org/10.1089/hyb.1990.9.63