Monoclonal antibodies to human alphafetoprotein

Marjatta Uotila; Eva Engvall; Erkki Ruoslahti

doi:10.1016/0161-5890(80)90150-9

Monoclonal antibodies to human alphafetoprotein

Marjatta Uotila, Eva Engvall, Erkki Ruoslahti

Neurology & Neurotherapeutics

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Antibodies to human alphafetoprotein were produced by hybridization of NS1/1 mouse myeloma cells with spleen cells from mice immunized with AFP. Four cell lines producing anti-AFP were established by cloning of cultures positive for anti-AFP from 3 separate hybridizations. These antibodies were all of IgG₁ (k) type. Highly sensitive radioimmunoassays were established using these monoclonal anti-AFPs. These assays gave the same quantitative values for AFP in samples of serum and amniotic fluid as assays using conventional antisera. Our results demonstrate that monoclonal antibodies with promising performance in radioimmuno-assay can be obtained. Such antibodies may provide useful reagents in clinical assays and should also become useful tools in delineating the antigenic structure of the AFP molecule.

Original language	English (US)
Pages (from-to)	791-794
Number of pages	4
Journal	Molecular Immunology
Volume	17
Issue number	6
DOIs	https://doi.org/10.1016/0161-5890(80)90150-9
State	Published - Jun 1980

ASJC Scopus subject areas

Immunology
Molecular Biology

Access to Document

10.1016/0161-5890(80)90150-9

Cite this

@article{41675dbfd1784009a5b613ee3e5f7000,

title = "Monoclonal antibodies to human alphafetoprotein",

abstract = "Antibodies to human alphafetoprotein were produced by hybridization of NS1/1 mouse myeloma cells with spleen cells from mice immunized with AFP. Four cell lines producing anti-AFP were established by cloning of cultures positive for anti-AFP from 3 separate hybridizations. These antibodies were all of IgG1 (k) type. Highly sensitive radioimmunoassays were established using these monoclonal anti-AFPs. These assays gave the same quantitative values for AFP in samples of serum and amniotic fluid as assays using conventional antisera. Our results demonstrate that monoclonal antibodies with promising performance in radioimmuno-assay can be obtained. Such antibodies may provide useful reagents in clinical assays and should also become useful tools in delineating the antigenic structure of the AFP molecule.",

author = "Marjatta Uotila and Eva Engvall and Erkki Ruoslahti",

note = "Funding Information: This work was supported by grant CA 27460 from the National Cancer Institute, DHS.",

year = "1980",

month = jun,

doi = "10.1016/0161-5890(80)90150-9",

language = "English (US)",

volume = "17",

pages = "791--794",

journal = "Molecular Immunology",

issn = "0161-5890",

publisher = "Elsevier Limited",

number = "6",

}

TY - JOUR

T1 - Monoclonal antibodies to human alphafetoprotein

AU - Uotila, Marjatta

AU - Engvall, Eva

AU - Ruoslahti, Erkki

N1 - Funding Information: This work was supported by grant CA 27460 from the National Cancer Institute, DHS.

PY - 1980/6

Y1 - 1980/6

N2 - Antibodies to human alphafetoprotein were produced by hybridization of NS1/1 mouse myeloma cells with spleen cells from mice immunized with AFP. Four cell lines producing anti-AFP were established by cloning of cultures positive for anti-AFP from 3 separate hybridizations. These antibodies were all of IgG1 (k) type. Highly sensitive radioimmunoassays were established using these monoclonal anti-AFPs. These assays gave the same quantitative values for AFP in samples of serum and amniotic fluid as assays using conventional antisera. Our results demonstrate that monoclonal antibodies with promising performance in radioimmuno-assay can be obtained. Such antibodies may provide useful reagents in clinical assays and should also become useful tools in delineating the antigenic structure of the AFP molecule.

AB - Antibodies to human alphafetoprotein were produced by hybridization of NS1/1 mouse myeloma cells with spleen cells from mice immunized with AFP. Four cell lines producing anti-AFP were established by cloning of cultures positive for anti-AFP from 3 separate hybridizations. These antibodies were all of IgG1 (k) type. Highly sensitive radioimmunoassays were established using these monoclonal anti-AFPs. These assays gave the same quantitative values for AFP in samples of serum and amniotic fluid as assays using conventional antisera. Our results demonstrate that monoclonal antibodies with promising performance in radioimmuno-assay can be obtained. Such antibodies may provide useful reagents in clinical assays and should also become useful tools in delineating the antigenic structure of the AFP molecule.

UR - http://www.scopus.com/inward/record.url?scp=0018910888&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018910888&partnerID=8YFLogxK

U2 - 10.1016/0161-5890(80)90150-9

DO - 10.1016/0161-5890(80)90150-9

M3 - Article

C2 - 6159528

AN - SCOPUS:0018910888

SN - 0161-5890

VL - 17

SP - 791

EP - 794

JO - Molecular Immunology

JF - Molecular Immunology

IS - 6

ER -

Monoclonal antibodies to human alphafetoprotein

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this