Monocyte-T lymphocyte interaction for regulation of insulin receptors on the activated T lymphocyte

J. H. Helderman, R. Ayuso, J. Rosenstock, Philip Raskin

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

During activation the lymphocyte attains functional insulin receptors with precise regulation, a consequence of insulin concentration manipulations. These studies test the hypothesis that insulin receptor (+) monocytes monitor insulin concentrations, so instructing the T lymphocytes. Monocyte-enriched populations were incubated with insulin (0-16-6 M) followed by co-culture with T lymphocytes and an activating stimulus. A dose-related fall in T lymphocyte insulin receptor binding was observed that was specific for the monocyte as the signalling cell and for insulin as the signal received. Monocytes from normal volunteers during a euglycemic, hyperinsulinemic clamp were cultured with T lymphocytes and an activating stimulus. A decline in specific insulin receptor binding on T lymphocytes was observed, which Scatchard analysis demonstrated to be a consequence of reduction in receptor numbers. These studies demonstrate that the receptor (+) monocyte perceives the concentration of insulin and passes this information to T lymphocytes regulating the number of activation-induced insulin receptors. The interplay between the monocyte and T lymphocyte parallels the interaction of these cell type for recognition of antigen.

Original languageEnglish (US)
Pages (from-to)566-571
Number of pages6
JournalJournal of Clinical Investigation
Volume79
Issue number2
StatePublished - 1987

Fingerprint

Insulin Receptor
Monocytes
T-Lymphocytes
Insulin
Glucose Clamp Technique
Lymphocyte Count
Lymphocyte Activation
Coculture Techniques
Cell Communication
Healthy Volunteers
Antigens
Population

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Monocyte-T lymphocyte interaction for regulation of insulin receptors on the activated T lymphocyte. / Helderman, J. H.; Ayuso, R.; Rosenstock, J.; Raskin, Philip.

In: Journal of Clinical Investigation, Vol. 79, No. 2, 1987, p. 566-571.

Research output: Contribution to journalArticle

@article{ab382c046eb04c299410e1cd31955c51,
title = "Monocyte-T lymphocyte interaction for regulation of insulin receptors on the activated T lymphocyte",
abstract = "During activation the lymphocyte attains functional insulin receptors with precise regulation, a consequence of insulin concentration manipulations. These studies test the hypothesis that insulin receptor (+) monocytes monitor insulin concentrations, so instructing the T lymphocytes. Monocyte-enriched populations were incubated with insulin (0-16-6 M) followed by co-culture with T lymphocytes and an activating stimulus. A dose-related fall in T lymphocyte insulin receptor binding was observed that was specific for the monocyte as the signalling cell and for insulin as the signal received. Monocytes from normal volunteers during a euglycemic, hyperinsulinemic clamp were cultured with T lymphocytes and an activating stimulus. A decline in specific insulin receptor binding on T lymphocytes was observed, which Scatchard analysis demonstrated to be a consequence of reduction in receptor numbers. These studies demonstrate that the receptor (+) monocyte perceives the concentration of insulin and passes this information to T lymphocytes regulating the number of activation-induced insulin receptors. The interplay between the monocyte and T lymphocyte parallels the interaction of these cell type for recognition of antigen.",
author = "Helderman, {J. H.} and R. Ayuso and J. Rosenstock and Philip Raskin",
year = "1987",
language = "English (US)",
volume = "79",
pages = "566--571",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "2",

}

TY - JOUR

T1 - Monocyte-T lymphocyte interaction for regulation of insulin receptors on the activated T lymphocyte

AU - Helderman, J. H.

AU - Ayuso, R.

AU - Rosenstock, J.

AU - Raskin, Philip

PY - 1987

Y1 - 1987

N2 - During activation the lymphocyte attains functional insulin receptors with precise regulation, a consequence of insulin concentration manipulations. These studies test the hypothesis that insulin receptor (+) monocytes monitor insulin concentrations, so instructing the T lymphocytes. Monocyte-enriched populations were incubated with insulin (0-16-6 M) followed by co-culture with T lymphocytes and an activating stimulus. A dose-related fall in T lymphocyte insulin receptor binding was observed that was specific for the monocyte as the signalling cell and for insulin as the signal received. Monocytes from normal volunteers during a euglycemic, hyperinsulinemic clamp were cultured with T lymphocytes and an activating stimulus. A decline in specific insulin receptor binding on T lymphocytes was observed, which Scatchard analysis demonstrated to be a consequence of reduction in receptor numbers. These studies demonstrate that the receptor (+) monocyte perceives the concentration of insulin and passes this information to T lymphocytes regulating the number of activation-induced insulin receptors. The interplay between the monocyte and T lymphocyte parallels the interaction of these cell type for recognition of antigen.

AB - During activation the lymphocyte attains functional insulin receptors with precise regulation, a consequence of insulin concentration manipulations. These studies test the hypothesis that insulin receptor (+) monocytes monitor insulin concentrations, so instructing the T lymphocytes. Monocyte-enriched populations were incubated with insulin (0-16-6 M) followed by co-culture with T lymphocytes and an activating stimulus. A dose-related fall in T lymphocyte insulin receptor binding was observed that was specific for the monocyte as the signalling cell and for insulin as the signal received. Monocytes from normal volunteers during a euglycemic, hyperinsulinemic clamp were cultured with T lymphocytes and an activating stimulus. A decline in specific insulin receptor binding on T lymphocytes was observed, which Scatchard analysis demonstrated to be a consequence of reduction in receptor numbers. These studies demonstrate that the receptor (+) monocyte perceives the concentration of insulin and passes this information to T lymphocytes regulating the number of activation-induced insulin receptors. The interplay between the monocyte and T lymphocyte parallels the interaction of these cell type for recognition of antigen.

UR - http://www.scopus.com/inward/record.url?scp=0023092399&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023092399&partnerID=8YFLogxK

M3 - Article

VL - 79

SP - 566

EP - 571

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 2

ER -