Moraxella catarrhalis expresses an unusual Hfq protein

Ahmed S. Attia, Jennifer L. Sedillo, Wei Wang, Wei Liu, Chad A Brautigam, Wade Winkler, Eric J Hansen

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27 Scopus citations

Abstract

The Hfq protein is recognized as a global regulatory molecule that facilitates certain RNA-RNA interactions in bacteria. BLAST analysis identified a 630-nucleotide open reading frame in the genome of Moraxella catarrhalis ATCC 43617 that was highly conserved among M. catarrhalis strains and which encoded a predicted protein with significant homology to the Hfq protein of Escherichia coli. This protein, containing 210 amino acids, was more than twice as large as the Hfq proteins previously described for other bacteria. The C-terminal half of the M. catarrhalis Hfq protein was very hydrophilic and contained two different types of amino acid repeats. A mutation in the M. catarrhalis hfq gene affected both the growth rate of this organism and its sensitivity to at least two different types of stress in vitro. Provision of the wild-type M. catarrhalis hfq gene in trans eliminated these phenotypic differences in the hfq mutant. This M. catarrhalis hfq mutant exhibited altered expression of some cell envelope proteins relative to the wild-type parent strain and also had a growth advantage in a continuous flow biofilm system. The presence of the wild-type M. catarrhalis hfq gene in trans in an E. coli hfq mutant fully reversed the modest growth deficiency of this E. coli mutant and partially reversed the stress sensitivity of this E. coli mutant to methyl viologen. The use of an electrophoretic mobility shift assay showed that this M. catarrhalis Hfq protein could bind RNA derived from a gene whose expression was altered in the M. catarrhalis hfq mutant.

Original languageEnglish (US)
Pages (from-to)2520-2530
Number of pages11
JournalInfection and immunity
Volume76
Issue number6
DOIs
StatePublished - Jun 2008

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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    Attia, A. S., Sedillo, J. L., Wang, W., Liu, W., Brautigam, C. A., Winkler, W., & Hansen, E. J. (2008). Moraxella catarrhalis expresses an unusual Hfq protein. Infection and immunity, 76(6), 2520-2530. https://doi.org/10.1128/IAI.01652-07