Morphologic effects of glycerol and urea on cochlear tissues of the chinchilla

C. G. Wright, W. L. Meyerhoff, D. H. Lee, P. S. Roland

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Glycerol and urea are used as test agents in confirming the diagnosis of endolymphatic hydrops. Although both substances act as osmotic diuretics, recent evidence suggests that they may have differing physiologic effects on the inner ear. This study was designed to compare the morphologic effects of urea and glycerol on cochlear tissues, using the chinchilla as an experimental model. Animals were given subcutaneous injections of glycerol (2 g/kg) or urea (1.2 g/kg) over periods of 3 hours, 4 days, or 1 week. Both agents were found to produce ultrastructural changes, including spiral ligament vacuolization, intracellular alterations of the stria vascularis, and increased numbers of Hensen's bodies in outer hair cells. These alterations appeared indicative of metabolic stress, but not toxicity. The morphologic findings provided no evidence that glycerol and urea affect the inner ear by fundamentally different mechanisms of action.

Original languageEnglish (US)
Pages (from-to)67-73
Number of pages7
JournalAnnals of Otology, Rhinology and Laryngology
Volume97
Issue number1
StatePublished - 1988

Fingerprint

Chinchilla
Cochlea
Glycerol
Urea
Inner Ear
Outer Auditory Hair Cells
Osmotic Diuretics
Spiral Ligament of Cochlea
Endolymphatic Hydrops
Stria Vascularis
Physiological Stress
Subcutaneous Injections
Theoretical Models

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Wright, C. G., Meyerhoff, W. L., Lee, D. H., & Roland, P. S. (1988). Morphologic effects of glycerol and urea on cochlear tissues of the chinchilla. Annals of Otology, Rhinology and Laryngology, 97(1), 67-73.

Morphologic effects of glycerol and urea on cochlear tissues of the chinchilla. / Wright, C. G.; Meyerhoff, W. L.; Lee, D. H.; Roland, P. S.

In: Annals of Otology, Rhinology and Laryngology, Vol. 97, No. 1, 1988, p. 67-73.

Research output: Contribution to journalArticle

Wright, CG, Meyerhoff, WL, Lee, DH & Roland, PS 1988, 'Morphologic effects of glycerol and urea on cochlear tissues of the chinchilla', Annals of Otology, Rhinology and Laryngology, vol. 97, no. 1, pp. 67-73.
Wright, C. G. ; Meyerhoff, W. L. ; Lee, D. H. ; Roland, P. S. / Morphologic effects of glycerol and urea on cochlear tissues of the chinchilla. In: Annals of Otology, Rhinology and Laryngology. 1988 ; Vol. 97, No. 1. pp. 67-73.
@article{f1025c99e7284a7ea63043dd030a896e,
title = "Morphologic effects of glycerol and urea on cochlear tissues of the chinchilla",
abstract = "Glycerol and urea are used as test agents in confirming the diagnosis of endolymphatic hydrops. Although both substances act as osmotic diuretics, recent evidence suggests that they may have differing physiologic effects on the inner ear. This study was designed to compare the morphologic effects of urea and glycerol on cochlear tissues, using the chinchilla as an experimental model. Animals were given subcutaneous injections of glycerol (2 g/kg) or urea (1.2 g/kg) over periods of 3 hours, 4 days, or 1 week. Both agents were found to produce ultrastructural changes, including spiral ligament vacuolization, intracellular alterations of the stria vascularis, and increased numbers of Hensen's bodies in outer hair cells. These alterations appeared indicative of metabolic stress, but not toxicity. The morphologic findings provided no evidence that glycerol and urea affect the inner ear by fundamentally different mechanisms of action.",
author = "Wright, {C. G.} and Meyerhoff, {W. L.} and Lee, {D. H.} and Roland, {P. S.}",
year = "1988",
language = "English (US)",
volume = "97",
pages = "67--73",
journal = "Annals of Otology, Rhinology and Laryngology",
issn = "0003-4894",
publisher = "Annals Publishing Company",
number = "1",

}

TY - JOUR

T1 - Morphologic effects of glycerol and urea on cochlear tissues of the chinchilla

AU - Wright, C. G.

AU - Meyerhoff, W. L.

AU - Lee, D. H.

AU - Roland, P. S.

PY - 1988

Y1 - 1988

N2 - Glycerol and urea are used as test agents in confirming the diagnosis of endolymphatic hydrops. Although both substances act as osmotic diuretics, recent evidence suggests that they may have differing physiologic effects on the inner ear. This study was designed to compare the morphologic effects of urea and glycerol on cochlear tissues, using the chinchilla as an experimental model. Animals were given subcutaneous injections of glycerol (2 g/kg) or urea (1.2 g/kg) over periods of 3 hours, 4 days, or 1 week. Both agents were found to produce ultrastructural changes, including spiral ligament vacuolization, intracellular alterations of the stria vascularis, and increased numbers of Hensen's bodies in outer hair cells. These alterations appeared indicative of metabolic stress, but not toxicity. The morphologic findings provided no evidence that glycerol and urea affect the inner ear by fundamentally different mechanisms of action.

AB - Glycerol and urea are used as test agents in confirming the diagnosis of endolymphatic hydrops. Although both substances act as osmotic diuretics, recent evidence suggests that they may have differing physiologic effects on the inner ear. This study was designed to compare the morphologic effects of urea and glycerol on cochlear tissues, using the chinchilla as an experimental model. Animals were given subcutaneous injections of glycerol (2 g/kg) or urea (1.2 g/kg) over periods of 3 hours, 4 days, or 1 week. Both agents were found to produce ultrastructural changes, including spiral ligament vacuolization, intracellular alterations of the stria vascularis, and increased numbers of Hensen's bodies in outer hair cells. These alterations appeared indicative of metabolic stress, but not toxicity. The morphologic findings provided no evidence that glycerol and urea affect the inner ear by fundamentally different mechanisms of action.

UR - http://www.scopus.com/inward/record.url?scp=0023841553&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023841553&partnerID=8YFLogxK

M3 - Article

VL - 97

SP - 67

EP - 73

JO - Annals of Otology, Rhinology and Laryngology

JF - Annals of Otology, Rhinology and Laryngology

SN - 0003-4894

IS - 1

ER -