Morphological and electrophysiological characterization of abnormal cell types in pediatric cortical dysplasia

Carlos Cepeda, Raymond S. Hurst, Jorge Flores-Hernández, Elizabeth Hernández-Echeagaray, Gloria J. Klapstein, Marea K. Boylan, Christopher R. Calvert, Emily L. Jocoy, Oanh K. Nguyen, Véronique M. André, Harry V. Vinters, Marjorie A. Ariano, Michael S. Levine, Gary W. Mathern

Research output: Contribution to journalArticle

137 Scopus citations

Abstract

The mechanisms responsible for seizure generation in cortical dysplasia (CD) are unknown, but morphologically abnormal cells could contribute. We examined the passive and active membrane properties of cells from pediatric CD in vitro. Normal- and abnormal-appearing cells were identified morphologically by using infrared videomicroscopy and biocytin in slices from children with mild to severe CD. Electrophysiological properties were assessed with patch clamp recordings. Four groups of abnormal -appearing cells were observed. The first consisted of large, pyramidal cells probably corresponding to cytomegalic neurons. Under conditions that reduced the contribution of K+ conductances, these cells generated large Ca2+ currents and influx when depolarized. When these cells were acutely dissociated, peak Ca2+ currents and densities were greater in cytomegalic compared with normal -appearing pyramidal neurons. The second group included large, nonpyramidal cells with atypical somatodendritic morphology that could correspond to "balloon" cells. These cells did not display active voltage- or ligand-gated currents and did not appear to receive synaptic inputs. The third group included misoriented and dysmorphic pyramidal neurons, and the fourth group consisted of immaturelooking pyramidal neurons. Electrophysiologically, neurons in these latter two groups did not display significant abnormalities when compared with normal-appearing pyramidal neurons. We conclude that there are cells with abnormal intrinsic membrane properties in pediatric CD. Among the four groups of cells, the most abnormal electrophysiological properties were displayed by cytomegalic neurons and large cells with atypical morphology. Cytomegalic neurons could play an important role in the generation of epileptic activity.

Original languageEnglish (US)
Pages (from-to)472-486
Number of pages15
JournalJournal of Neuroscience Research
Volume72
Issue number4
DOIs
StatePublished - May 15 2003

    Fingerprint

Keywords

  • Calcium imaging
  • Cortical dysplasia
  • Cytomegalic neurons
  • Electrophysiology
  • Epilepsy

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Cepeda, C., Hurst, R. S., Flores-Hernández, J., Hernández-Echeagaray, E., Klapstein, G. J., Boylan, M. K., Calvert, C. R., Jocoy, E. L., Nguyen, O. K., André, V. M., Vinters, H. V., Ariano, M. A., Levine, M. S., & Mathern, G. W. (2003). Morphological and electrophysiological characterization of abnormal cell types in pediatric cortical dysplasia. Journal of Neuroscience Research, 72(4), 472-486. https://doi.org/10.1002/jnr.10604