Morphological, ultrastructural, and genetic characterization of an unusual T-cell lymphoma in a patient with sinus histiocytosis with massive lymphadenopathy

P. R K Koduru, U. Susin, J. E. Kolitz, M. Soni, S. Teichberg, M. J. Siques, T. Sun, E. Amorosi, D. R. Budman

Research output: Contribution to journalArticle

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Abstract

Sinus histiocytosis with massive lymphadenopathy (SHML) is a rare benign disease of unknown etiology. It is rarely associated with malignant lymphoma. This report documents the first case of a T-cell lymphoma, which developed in a patient with a 10-year history of SHML. The disease was complicated by hypereosinophilia and massive retroperitoneal lymphadenopathy. Histological examination of a cervical lymph node biopsy during the terminal phase identified a lymphoma composed of cells with morphological plasmacytoid features. Ultrastructurally, the tumor cells showed poorly developed cytoplasm, nuclei with peripheral chromatin clumping, and inconspicuous nucleoli. Cytogenetic studies showed two related clones. On immunohistochemical staining tumor cells were positive with monoclonal antibodies (mAb) CD3 and CD45RO. Southern blotting analysis identified clonal rearrangements in the T-cell receptor (TCR) alpha, beta and gamma genes. Thus, T-cell lineage of the tumor cells was established. In situ hybridization of interleukin-2 (IL-2) and interleukin-5 (IL-5) cDNA probes on tissue sections identified the synthesis of IL-5 by the eosinophils, suggesting an autocrine pathway of eosinophilopoiesis leading to hypereosinophilia in this patient.

Original languageEnglish (US)
Pages (from-to)192-200
Number of pages9
JournalAmerican Journal of Hematology
Volume48
Issue number3
DOIs
StatePublished - 1995

Fingerprint

Sinus Histiocytosis
T-Cell Lymphoma
Interleukin-5
Lymphoma
T-Cell Receptor alpha Genes
T-Cell Receptor gamma Genes
T-Cell Receptor beta Genes
Neoplasms
Cell Lineage
Rare Diseases
Southern Blotting
Eosinophils
Cytogenetics
Chromatin
Interleukin-2
In Situ Hybridization
Cytoplasm
Complementary DNA
Clone Cells
Lymph Nodes

Keywords

  • Molecular genetics
  • Sinus histiocytosis with massive lymphadenopathy
  • T-cell lymphoma
  • Ultrastructure

ASJC Scopus subject areas

  • Hematology

Cite this

Morphological, ultrastructural, and genetic characterization of an unusual T-cell lymphoma in a patient with sinus histiocytosis with massive lymphadenopathy. / Koduru, P. R K; Susin, U.; Kolitz, J. E.; Soni, M.; Teichberg, S.; Siques, M. J.; Sun, T.; Amorosi, E.; Budman, D. R.

In: American Journal of Hematology, Vol. 48, No. 3, 1995, p. 192-200.

Research output: Contribution to journalArticle

Koduru, P. R K ; Susin, U. ; Kolitz, J. E. ; Soni, M. ; Teichberg, S. ; Siques, M. J. ; Sun, T. ; Amorosi, E. ; Budman, D. R. / Morphological, ultrastructural, and genetic characterization of an unusual T-cell lymphoma in a patient with sinus histiocytosis with massive lymphadenopathy. In: American Journal of Hematology. 1995 ; Vol. 48, No. 3. pp. 192-200.
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