Morphology of a 'noninfectious' sarcoma virus

W. T. Hall, A. F. Gazdar, B. A. Hobbs, H. C. Chopra

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Two virus producing cell culture lines, HTG2 and HTG3, were established from a transplantable hamster tumor induced by a murine sarcoma virus (MSV) after 17 and 60 in vivo passages, respectively. HTG2 cells presumably produced only noninfectious sarcoma virions, whereas HTG3 cells yielded both sarcoma and 'helper' viruses. Electron microscopic examination of the original virus induced tumors, of pellets from cells grown in tissue culture, and of tissue culture medium harvested at 24, 48, 72, and 96 hr consistently revealed the same morphologic characteristics: More than 99% of the HTG2 virions retained their electron lucent or 'immature' form, in marked contrast to HTG3 virions or other typical type C viruses, which formed electron dense nucleoids. In addition, a large percentage of both HTG2 and HTG3 particles appeared morphologically incomplete, though completely detached or released from the cells. The inability to form dense nucleoids may be characteristic of noninfectious murine sarcoma viruses.

Original languageEnglish (US)
Pages (from-to)1337-1343
Number of pages7
JournalJournal of the National Cancer Institute
Volume52
Issue number4
StatePublished - 1974

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Sarcoma
Murine Sarcoma Viruses
Virion
Viruses
Electrons
Helper Viruses
Oncogenic Viruses
Cricetinae
Culture Media
Cell Culture Techniques
Cell Line
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Hall, W. T., Gazdar, A. F., Hobbs, B. A., & Chopra, H. C. (1974). Morphology of a 'noninfectious' sarcoma virus. Journal of the National Cancer Institute, 52(4), 1337-1343.

Morphology of a 'noninfectious' sarcoma virus. / Hall, W. T.; Gazdar, A. F.; Hobbs, B. A.; Chopra, H. C.

In: Journal of the National Cancer Institute, Vol. 52, No. 4, 1974, p. 1337-1343.

Research output: Contribution to journalArticle

Hall, WT, Gazdar, AF, Hobbs, BA & Chopra, HC 1974, 'Morphology of a 'noninfectious' sarcoma virus', Journal of the National Cancer Institute, vol. 52, no. 4, pp. 1337-1343.
Hall WT, Gazdar AF, Hobbs BA, Chopra HC. Morphology of a 'noninfectious' sarcoma virus. Journal of the National Cancer Institute. 1974;52(4):1337-1343.
Hall, W. T. ; Gazdar, A. F. ; Hobbs, B. A. ; Chopra, H. C. / Morphology of a 'noninfectious' sarcoma virus. In: Journal of the National Cancer Institute. 1974 ; Vol. 52, No. 4. pp. 1337-1343.
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