Mouse DNA polymerase kappa has a functional role in the repair of DNA strand breaks

Xiuli Zhang, Lingna Lv, Qian Chen, Fenghua Yuan, Ting Zhang, Yeran Yang, Hui Zhang, Yun Wang, Yan Jia, Liangyue Qian, Benjamin Chen, Yanbin Zhang, Errol C. Friedberg, Tie Shan Tang, Caixia Guo

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The Y-family of DNA polymerases support of translesion DNA synthesis (TLS) associated with stalled DNA replication by DNA damage. Recently, a number of studies suggest that some specialized TLS polymerases also support other aspects of DNA metabolism beyond TLS in vivo. Here we show that mouse polymerase kappa (Polκ) could accumulate at laser-induced sites of damage in vivo resembling polymerases eta and iota. The recruitment was mediated through Polκ C-terminus which contains the PCNA-interacting peptide, ubiquitin zinc finger motif 2 and nuclear localization signal. Interestingly, this recruitment was significantly reduced in MSH2-deficient LoVo cells and Rad18-depleted cells. We further observed that Polκ-deficient mouse embryo fibroblasts were abnormally sensitive to H2O2 treatment and displayed defects in both single-strand break repair and double-strand break repair. We speculate that Polκ may have an important role in strand break repair following oxidative stress in vivo.

Original languageEnglish (US)
Pages (from-to)377-388
Number of pages12
JournalDNA repair
Volume12
Issue number5
DOIs
StatePublished - May 1 2013

Keywords

  • Laser micro-irradiation
  • MSH2
  • PCNA
  • Polymerase kappa
  • Strand break repair

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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