Mouse extrahepatic hepatoma detected on microPET using copper (II)-64 chloride uptake mediated by endogenous mouse copper transporter 1

Fangyu Peng, Jianguo Liu, Jiu Sheng Wu, Xin Lu, Otto Muzik

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Purpose: This study was conducted to assess the positron-emitting copper (II)-64 chloride (64CuCl2) as a probe for imaging mouse extrahepatic hepatoma expressing mouse copper transporter 1 (mCtr1) with positron emission tomography (PET). Procedures: Following the intravenous administration of 64CuCl2, athymic mice bearing extrahepatic hepatoma grafts were subjected to whole-body static PET imaging with a Concorde microPET R4 tomograph. Upon completion of the imaging study, immunohistochemistry (IHC) study of mCtr1 was performed with postmortem tissues. Results: The mouse extrahepatic hepatoma grafts were well visualized on static microPET images. Quantitative analysis demonstrated that the tracer concentration in the hepatoma was significantly higher than those in the soft tissue of the right shoulder opposite to the tumor site and the brain (p < 0.001). mCtr1 immunoreactivity in the hepatoma graft was approximately 70% of that in liver, whereas 64CuCl2 concentration in the graft was approximately 11% of the liver concentration. Conclusions: The extrahepatic mouse hepatoma grafts may be visualized by Cu-64 PET, taking advantage of the 64CuCl2 uptake mediated by the functional endogenous mCtr1.

Original languageEnglish (US)
Pages (from-to)325-329
Number of pages5
JournalMolecular Imaging and Biology
Volume7
Issue number5
DOIs
StatePublished - Sep 2005

Fingerprint

Hepatocellular Carcinoma
Transplants
Positron-Emission Tomography
Liver
Nude Mice
Brain Neoplasms
Intravenous Administration
Mouse Ctr1 protein
cupric chloride
Immunohistochemistry
Electrons

Keywords

  • Copper (II)-64 chloride
  • Human hepatocellular carcinoma
  • Molecular imaging
  • Mouse copper transporter 1
  • Mouse hepatoma
  • Positron emission tomography

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Radiology Nuclear Medicine and imaging

Cite this

Mouse extrahepatic hepatoma detected on microPET using copper (II)-64 chloride uptake mediated by endogenous mouse copper transporter 1. / Peng, Fangyu; Liu, Jianguo; Wu, Jiu Sheng; Lu, Xin; Muzik, Otto.

In: Molecular Imaging and Biology, Vol. 7, No. 5, 09.2005, p. 325-329.

Research output: Contribution to journalArticle

@article{beb86e633213417f8b8cf511f73df6b4,
title = "Mouse extrahepatic hepatoma detected on microPET using copper (II)-64 chloride uptake mediated by endogenous mouse copper transporter 1",
abstract = "Purpose: This study was conducted to assess the positron-emitting copper (II)-64 chloride (64CuCl2) as a probe for imaging mouse extrahepatic hepatoma expressing mouse copper transporter 1 (mCtr1) with positron emission tomography (PET). Procedures: Following the intravenous administration of 64CuCl2, athymic mice bearing extrahepatic hepatoma grafts were subjected to whole-body static PET imaging with a Concorde microPET R4 tomograph. Upon completion of the imaging study, immunohistochemistry (IHC) study of mCtr1 was performed with postmortem tissues. Results: The mouse extrahepatic hepatoma grafts were well visualized on static microPET images. Quantitative analysis demonstrated that the tracer concentration in the hepatoma was significantly higher than those in the soft tissue of the right shoulder opposite to the tumor site and the brain (p < 0.001). mCtr1 immunoreactivity in the hepatoma graft was approximately 70{\%} of that in liver, whereas 64CuCl2 concentration in the graft was approximately 11{\%} of the liver concentration. Conclusions: The extrahepatic mouse hepatoma grafts may be visualized by Cu-64 PET, taking advantage of the 64CuCl2 uptake mediated by the functional endogenous mCtr1.",
keywords = "Copper (II)-64 chloride, Human hepatocellular carcinoma, Molecular imaging, Mouse copper transporter 1, Mouse hepatoma, Positron emission tomography",
author = "Fangyu Peng and Jianguo Liu and Wu, {Jiu Sheng} and Xin Lu and Otto Muzik",
year = "2005",
month = "9",
doi = "10.1007/s11307-005-0021-4",
language = "English (US)",
volume = "7",
pages = "325--329",
journal = "Molecular Imaging and Biology",
issn = "1536-1632",
publisher = "Springer New York",
number = "5",

}

TY - JOUR

T1 - Mouse extrahepatic hepatoma detected on microPET using copper (II)-64 chloride uptake mediated by endogenous mouse copper transporter 1

AU - Peng, Fangyu

AU - Liu, Jianguo

AU - Wu, Jiu Sheng

AU - Lu, Xin

AU - Muzik, Otto

PY - 2005/9

Y1 - 2005/9

N2 - Purpose: This study was conducted to assess the positron-emitting copper (II)-64 chloride (64CuCl2) as a probe for imaging mouse extrahepatic hepatoma expressing mouse copper transporter 1 (mCtr1) with positron emission tomography (PET). Procedures: Following the intravenous administration of 64CuCl2, athymic mice bearing extrahepatic hepatoma grafts were subjected to whole-body static PET imaging with a Concorde microPET R4 tomograph. Upon completion of the imaging study, immunohistochemistry (IHC) study of mCtr1 was performed with postmortem tissues. Results: The mouse extrahepatic hepatoma grafts were well visualized on static microPET images. Quantitative analysis demonstrated that the tracer concentration in the hepatoma was significantly higher than those in the soft tissue of the right shoulder opposite to the tumor site and the brain (p < 0.001). mCtr1 immunoreactivity in the hepatoma graft was approximately 70% of that in liver, whereas 64CuCl2 concentration in the graft was approximately 11% of the liver concentration. Conclusions: The extrahepatic mouse hepatoma grafts may be visualized by Cu-64 PET, taking advantage of the 64CuCl2 uptake mediated by the functional endogenous mCtr1.

AB - Purpose: This study was conducted to assess the positron-emitting copper (II)-64 chloride (64CuCl2) as a probe for imaging mouse extrahepatic hepatoma expressing mouse copper transporter 1 (mCtr1) with positron emission tomography (PET). Procedures: Following the intravenous administration of 64CuCl2, athymic mice bearing extrahepatic hepatoma grafts were subjected to whole-body static PET imaging with a Concorde microPET R4 tomograph. Upon completion of the imaging study, immunohistochemistry (IHC) study of mCtr1 was performed with postmortem tissues. Results: The mouse extrahepatic hepatoma grafts were well visualized on static microPET images. Quantitative analysis demonstrated that the tracer concentration in the hepatoma was significantly higher than those in the soft tissue of the right shoulder opposite to the tumor site and the brain (p < 0.001). mCtr1 immunoreactivity in the hepatoma graft was approximately 70% of that in liver, whereas 64CuCl2 concentration in the graft was approximately 11% of the liver concentration. Conclusions: The extrahepatic mouse hepatoma grafts may be visualized by Cu-64 PET, taking advantage of the 64CuCl2 uptake mediated by the functional endogenous mCtr1.

KW - Copper (II)-64 chloride

KW - Human hepatocellular carcinoma

KW - Molecular imaging

KW - Mouse copper transporter 1

KW - Mouse hepatoma

KW - Positron emission tomography

UR - http://www.scopus.com/inward/record.url?scp=33644897850&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644897850&partnerID=8YFLogxK

U2 - 10.1007/s11307-005-0021-4

DO - 10.1007/s11307-005-0021-4

M3 - Article

C2 - 16220354

AN - SCOPUS:33644897850

VL - 7

SP - 325

EP - 329

JO - Molecular Imaging and Biology

JF - Molecular Imaging and Biology

SN - 1536-1632

IS - 5

ER -