Mouse models to study natural killer cell-mediated immunosurveillance and metastatic latency

Vidhya R. Nair, Srinivas Malladi

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Metastatic latency is a major concern in the clinic, yet how these disseminated cancer cells survive and initiate metastases is unknown (Massagué and Obenauf, Nature 529:298–306, 2016). Here, we describe an approach to isolate latency competent cancer (LCC) cells from early stage human lung and breast carcinoma cell lines using mouse xenograft models (Malladi, Cell 165:45–60, 2016). Cancer cell lines labeled with GFP-luciferase and antibiotic selection markers were injected intracardially into athymic mice. Three months, post-injection, LCC cells were identified in situ and isolated. Upon reinjection, LCC cells retain their tumorigenic potential, enter a slow-cycling or quiescent state, and evade NK cell-mediated innate immune surveillance.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages141-150
Number of pages10
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1884
ISSN (Print)1064-3745

Keywords

  • Cancer
  • Disseminated tumor cells
  • Dormancy
  • LCC cells
  • Metastasis
  • Metastatic latency
  • NK cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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