@inbook{c124370548d3410fa9ffe5f972924519,
title = "Mouse models to study natural killer cell-mediated immunosurveillance and metastatic latency",
abstract = "Metastatic latency is a major concern in the clinic, yet how these disseminated cancer cells survive and initiate metastases is unknown (Massagu{\'e} and Obenauf, Nature 529:298–306, 2016). Here, we describe an approach to isolate latency competent cancer (LCC) cells from early stage human lung and breast carcinoma cell lines using mouse xenograft models (Malladi, Cell 165:45–60, 2016). Cancer cell lines labeled with GFP-luciferase and antibiotic selection markers were injected intracardially into athymic mice. Three months, post-injection, LCC cells were identified in situ and isolated. Upon reinjection, LCC cells retain their tumorigenic potential, enter a slow-cycling or quiescent state, and evade NK cell-mediated innate immune surveillance.",
keywords = "Cancer, Disseminated tumor cells, Dormancy, LCC cells, Metastasis, Metastatic latency, NK cells",
author = "Nair, {Vidhya R.} and Srinivas Malladi",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-8885-3_9",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "141--150",
booktitle = "Methods in Molecular Biology",
}