Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis

Caixia Guo, Paula L. Fischhaber, Margaret J. Luk-Paszyc, Yuji Masuda, Jing Zhou, Kenji Kamiya, Caroline Kisker, Errol C. Friedberg

Research output: Contribution to journalArticle

259 Citations (Scopus)

Abstract

Polκ and Rev1 are members of the Y family of DNA polymerases involved in tolerance to DNA damage by replicative bypass [translesion DNA synthesis (TLS)]. We demonstrate that mouse Rev1 protein physically associates with Polκ. We show too that Rev1 interacts independently with Rev7 (a subunit of a TLS polymerase, Polζ) and with two other Y-family polymerases, Polι and Polη. Mouse Polκ, Rev7, Polι, and Polη each bind to the same ∼100 amino acid C-terminal region of Rev1. Furthermore, Rev7 competes directly with Polκ for binding to the Rev1 C-terminus. Notwithstanding the physical interaction between Rev1 and Polκ, the DNA polymerase activity of each measured by primer extension in vitro is unaffected by the complex, either when extending normal primer-termini, when bypassing a single thymine glycol lesion, or when extending certain mismatched primer termini. Our observations suggest that Rev1 plays a role(s) in mediating protein-protein interactions among DNA polymerases required for TLS. The precise function(s) of these interactions during TLS remains to be determined.

Original languageEnglish (US)
Pages (from-to)6621-6630
Number of pages10
JournalEMBO Journal
Volume22
Issue number24
DOIs
StatePublished - Dec 15 2003

Fingerprint

DNA-Directed DNA Polymerase
DNA
DNA Damage
Proteins
Amino Acids
Mouse Rev1l protein

Keywords

  • DNA polymerases
  • Mutagenesis
  • Polκ
  • Rev1
  • Translesion DNA synthesis

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Guo, C., Fischhaber, P. L., Luk-Paszyc, M. J., Masuda, Y., Zhou, J., Kamiya, K., ... Friedberg, E. C. (2003). Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis. EMBO Journal, 22(24), 6621-6630. https://doi.org/10.1093/emboj/cdg626

Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis. / Guo, Caixia; Fischhaber, Paula L.; Luk-Paszyc, Margaret J.; Masuda, Yuji; Zhou, Jing; Kamiya, Kenji; Kisker, Caroline; Friedberg, Errol C.

In: EMBO Journal, Vol. 22, No. 24, 15.12.2003, p. 6621-6630.

Research output: Contribution to journalArticle

Guo, C, Fischhaber, PL, Luk-Paszyc, MJ, Masuda, Y, Zhou, J, Kamiya, K, Kisker, C & Friedberg, EC 2003, 'Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis', EMBO Journal, vol. 22, no. 24, pp. 6621-6630. https://doi.org/10.1093/emboj/cdg626
Guo C, Fischhaber PL, Luk-Paszyc MJ, Masuda Y, Zhou J, Kamiya K et al. Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis. EMBO Journal. 2003 Dec 15;22(24):6621-6630. https://doi.org/10.1093/emboj/cdg626
Guo, Caixia ; Fischhaber, Paula L. ; Luk-Paszyc, Margaret J. ; Masuda, Yuji ; Zhou, Jing ; Kamiya, Kenji ; Kisker, Caroline ; Friedberg, Errol C. / Mouse Rev1 protein interacts with multiple DNA polymerases involved in translesion DNA synthesis. In: EMBO Journal. 2003 ; Vol. 22, No. 24. pp. 6621-6630.
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