Mouse spermatogenesis requires classical and nonclassical testosterone signaling

Corey Toocheck, Terri Clister, John Shupe, Chelsea Crum, Preethi Ravindranathan, Tae Kyung Lee, Jung Mo Ahn, Ganesh V. Raj, Meena Sukhwani, Kyle E. Orwig, William H. Walker

Research output: Contribution to journalArticlepeer-review

64 Scopus citations


Testosterone acts though the androgen receptor in Sertoli cells to support germ cell development (spermatogenesis) and male fertility, but the molecular and cellular mechanisms by which testosterone acts are not well understood. Previously, we found that in addition to acting through androgen receptor to directly regulate gene expression (classical testosterone signaling pathway), testosterone acts through a nonclassical pathway via the androgen receptor to rapidly activate kinases that are known to regulate spermatogenesis. In this study, we provide the first evidence that nonclassical testosterone signaling occurs in vivo as the MAP kinase cascade is rapidly activated in Sertoli cells within the testis by increasing testosterone levels in the rat. We find that either classical or nonclassical signaling regulates testosterone-mediated Rhox5 gene expression in Sertoli cells within testis explants. The selective activation of classical or nonclassical signaling pathways in Sertoli cells within testis explants also resulted in the differential activation of the Zbtb16 and c-Kit genes in adjacent spermatogonia germ cells. Delivery of an inhibitor of either pathway to Sertoli cells of mouse testes disrupted the blood-testis barrier that is essential for spermatogenesis. Furthermore, an inhibitor of nonclassical testosterone signaling blocked meiosis in pubertal mice and caused the loss of meiotic and postmeiotic germ cells in adult mouse testes. An inhibitor of the classical pathway caused the premature release of immature germ cells. Collectively, these observations indicate that classical and nonclassical testosterone signaling regulate overlapping and distinct functions that are required for the maintenance of spermatogenesis and male fertility.

Original languageEnglish (US)
Article number11
JournalBiology of reproduction
Issue number1
StatePublished - Jan 1 2016


  • Androgen receptor
  • Fertility
  • Nongenomic
  • Sertoli cell
  • Testis

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology


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