Abstract
Piwi-interacting RNAs (piRNAs) comprise a broad class of small noncoding RNAs that function as an endogenous defense system against transposable elements. Here we show that the putative DExD-box helicase MOV10-like-1 (MOV10L1) is essential for silencing retrotransposons in the mouse male germline. Mov10l1 is specifically expressed in germ cells with increasing expression from gonocytes/type A spermatogonia to pachytene spermatocytes. Primary spermatocytes of Mov10l1-/- mice showactivation of LTR and LINE-1 retrotransposons, followed by cell death, causing male infertility and a complete block of spermatogenesis at early prophase of meiosis I. Despite the early expression of Mov10l1, germline stem cell maintenance appears unaffected in Mov10l1-/- mice. MOV10L1 interacts with the Piwi proteins MILI and MIWI.MOV10L1 also interacts with heat shock 70-kDa protein 2 (HSPA2), a testis-enriched chaperone expressed in pachytene spermatocytes and also essential for male fertility. These studies reveal a crucial role of MOV10L1 in male fertility and piRNA-directed retrotransposon silencing in male germ cells and suggest that MOV10L1 functions as a key component of a safeguard mechanism for the genetic information in male germ cells of mammals.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 11847-11852 |
| Number of pages | 6 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 107 |
| Issue number | 26 |
| DOIs | |
| State | Published - Jun 29 2010 |
Fingerprint
Keywords
- Armitage
- Meiosis
- RNA helicase
ASJC Scopus subject areas
- General
Cite this
MOV10L1 is necessary for protection of spermatocytes against retrotransposons by Piwi-interacting RNAs. / Frost, Robert J A; Hamra, F. Kent; Richardson, James A.; Qi, Xiaoxia; Bassel-Duby, Rhonda; Olson, Eric N.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 26, 29.06.2010, p. 11847-11852.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - MOV10L1 is necessary for protection of spermatocytes against retrotransposons by Piwi-interacting RNAs
AU - Frost, Robert J A
AU - Hamra, F. Kent
AU - Richardson, James A.
AU - Qi, Xiaoxia
AU - Bassel-Duby, Rhonda
AU - Olson, Eric N.
PY - 2010/6/29
Y1 - 2010/6/29
N2 - Piwi-interacting RNAs (piRNAs) comprise a broad class of small noncoding RNAs that function as an endogenous defense system against transposable elements. Here we show that the putative DExD-box helicase MOV10-like-1 (MOV10L1) is essential for silencing retrotransposons in the mouse male germline. Mov10l1 is specifically expressed in germ cells with increasing expression from gonocytes/type A spermatogonia to pachytene spermatocytes. Primary spermatocytes of Mov10l1-/- mice showactivation of LTR and LINE-1 retrotransposons, followed by cell death, causing male infertility and a complete block of spermatogenesis at early prophase of meiosis I. Despite the early expression of Mov10l1, germline stem cell maintenance appears unaffected in Mov10l1-/- mice. MOV10L1 interacts with the Piwi proteins MILI and MIWI.MOV10L1 also interacts with heat shock 70-kDa protein 2 (HSPA2), a testis-enriched chaperone expressed in pachytene spermatocytes and also essential for male fertility. These studies reveal a crucial role of MOV10L1 in male fertility and piRNA-directed retrotransposon silencing in male germ cells and suggest that MOV10L1 functions as a key component of a safeguard mechanism for the genetic information in male germ cells of mammals.
AB - Piwi-interacting RNAs (piRNAs) comprise a broad class of small noncoding RNAs that function as an endogenous defense system against transposable elements. Here we show that the putative DExD-box helicase MOV10-like-1 (MOV10L1) is essential for silencing retrotransposons in the mouse male germline. Mov10l1 is specifically expressed in germ cells with increasing expression from gonocytes/type A spermatogonia to pachytene spermatocytes. Primary spermatocytes of Mov10l1-/- mice showactivation of LTR and LINE-1 retrotransposons, followed by cell death, causing male infertility and a complete block of spermatogenesis at early prophase of meiosis I. Despite the early expression of Mov10l1, germline stem cell maintenance appears unaffected in Mov10l1-/- mice. MOV10L1 interacts with the Piwi proteins MILI and MIWI.MOV10L1 also interacts with heat shock 70-kDa protein 2 (HSPA2), a testis-enriched chaperone expressed in pachytene spermatocytes and also essential for male fertility. These studies reveal a crucial role of MOV10L1 in male fertility and piRNA-directed retrotransposon silencing in male germ cells and suggest that MOV10L1 functions as a key component of a safeguard mechanism for the genetic information in male germ cells of mammals.
KW - Armitage
KW - Meiosis
KW - RNA helicase
UR - http://www.scopus.com/inward/record.url?scp=77955409741&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955409741&partnerID=8YFLogxK
U2 - 10.1073/pnas.1007158107
DO - 10.1073/pnas.1007158107
M3 - Article
C2 - 20547853
AN - SCOPUS:77955409741
VL - 107
SP - 11847
EP - 11852
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 26
ER -