MRI and histopathologic study of a novel cholesterol-fed rabbit model of xanthogranuloma

Yuanxin Chen, Amanda M. Hamilton, Katie M. Parkins, Jian Xiong Wang, Kem A. Rogers, Michael M. Zeineh, Brian K. Rutt, John A. Ronald

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose: To develop a rabbit model of xanthogranuloma based on supplementation of dietary cholesterol. The aim of this study was to analyze the xanthogranulomatous lesions using magnetic resonance imaging (MRI) and histological examination. Materials and Methods: Rabbits were fed a low-level cholesterol (CH) diet (n = 10) or normal chow (n = 5) for 24 months. In vivo brain imaging was performed on a 3T MR system using fast imaging employing steady state acquisition, susceptibility-weighted imaging, spoiled gradient recalled, T1-weighted inversion recovery imaging and T1 relaxometry, PD-weighted and T2-weighted spin-echo imaging and T2 relaxometry, iterative decomposition of water and fat with echo asymmetry and least-squares estimation, ultrashort TE MRI (UTE-MRI), and T2* relaxometry. MR images were evaluated using a Likert scale for lesion presence and quantitative analysis of lesion size, ventricular volume, and T1, T2, and T2* values of lesions was performed. After imaging, brain specimens were examined using histological methods. Results: In vivo MRI revealed that 6 of 10 CH-fed rabbits developed lesions in the choroid plexus. Region-of-interest analysis showed that for CH-fed rabbits the mean lesion volume was 8.5 ± 2.6 mm3 and the volume of the lateral ventricle was significantly increased compared to controls (P < 0.01). The lesions showed significantly shorter mean T2 values (35 ± 12 msec, P < 0.001), longer mean T1 values (1581 ± 146 msec, P < 0.05), and shorter T2* values (22 ± 13 msec, P < 0.001) compared to adjacent brain structures. The ultrashort T2* components were visible using UTE-MRI. Histopathologic evaluation of lesions demonstrated features of human xanthogranuloma. Conclusion: Rabbits fed a low-level CH diet develop sizable intraventricular masses that have similar histopathological features as human xanthogranuloma. Multiparametric MRI techniques were able to provide information about the complex composition of these lesions. J. Magn. Reson. Imaging 2016;44:673–682.

Original languageEnglish (US)
Pages (from-to)673-682
Number of pages10
JournalJournal of Magnetic Resonance Imaging
Volume44
Issue number3
DOIs
StatePublished - Sep 1 2016

Fingerprint

Cholesterol
Magnetic Resonance Imaging
Rabbits
Diet
Dietary Cholesterol
Choroid Plexus
Lateral Ventricles
Brain
Least-Squares Analysis
Neuroimaging
Fats
Water

Keywords

  • cholesterol
  • hypercholesterolemia
  • magnetic resonance imaging
  • rabbit model
  • xanthogranuloma

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Chen, Y., Hamilton, A. M., Parkins, K. M., Wang, J. X., Rogers, K. A., Zeineh, M. M., ... Ronald, J. A. (2016). MRI and histopathologic study of a novel cholesterol-fed rabbit model of xanthogranuloma. Journal of Magnetic Resonance Imaging, 44(3), 673-682. https://doi.org/10.1002/jmri.25213

MRI and histopathologic study of a novel cholesterol-fed rabbit model of xanthogranuloma. / Chen, Yuanxin; Hamilton, Amanda M.; Parkins, Katie M.; Wang, Jian Xiong; Rogers, Kem A.; Zeineh, Michael M.; Rutt, Brian K.; Ronald, John A.

In: Journal of Magnetic Resonance Imaging, Vol. 44, No. 3, 01.09.2016, p. 673-682.

Research output: Contribution to journalArticle

Chen, Y, Hamilton, AM, Parkins, KM, Wang, JX, Rogers, KA, Zeineh, MM, Rutt, BK & Ronald, JA 2016, 'MRI and histopathologic study of a novel cholesterol-fed rabbit model of xanthogranuloma', Journal of Magnetic Resonance Imaging, vol. 44, no. 3, pp. 673-682. https://doi.org/10.1002/jmri.25213
Chen, Yuanxin ; Hamilton, Amanda M. ; Parkins, Katie M. ; Wang, Jian Xiong ; Rogers, Kem A. ; Zeineh, Michael M. ; Rutt, Brian K. ; Ronald, John A. / MRI and histopathologic study of a novel cholesterol-fed rabbit model of xanthogranuloma. In: Journal of Magnetic Resonance Imaging. 2016 ; Vol. 44, No. 3. pp. 673-682.
@article{6a13fad714124f628308ec197b954636,
title = "MRI and histopathologic study of a novel cholesterol-fed rabbit model of xanthogranuloma",
abstract = "Purpose: To develop a rabbit model of xanthogranuloma based on supplementation of dietary cholesterol. The aim of this study was to analyze the xanthogranulomatous lesions using magnetic resonance imaging (MRI) and histological examination. Materials and Methods: Rabbits were fed a low-level cholesterol (CH) diet (n = 10) or normal chow (n = 5) for 24 months. In vivo brain imaging was performed on a 3T MR system using fast imaging employing steady state acquisition, susceptibility-weighted imaging, spoiled gradient recalled, T1-weighted inversion recovery imaging and T1 relaxometry, PD-weighted and T2-weighted spin-echo imaging and T2 relaxometry, iterative decomposition of water and fat with echo asymmetry and least-squares estimation, ultrashort TE MRI (UTE-MRI), and T2* relaxometry. MR images were evaluated using a Likert scale for lesion presence and quantitative analysis of lesion size, ventricular volume, and T1, T2, and T2* values of lesions was performed. After imaging, brain specimens were examined using histological methods. Results: In vivo MRI revealed that 6 of 10 CH-fed rabbits developed lesions in the choroid plexus. Region-of-interest analysis showed that for CH-fed rabbits the mean lesion volume was 8.5 ± 2.6 mm3 and the volume of the lateral ventricle was significantly increased compared to controls (P < 0.01). The lesions showed significantly shorter mean T2 values (35 ± 12 msec, P < 0.001), longer mean T1 values (1581 ± 146 msec, P < 0.05), and shorter T2* values (22 ± 13 msec, P < 0.001) compared to adjacent brain structures. The ultrashort T2* components were visible using UTE-MRI. Histopathologic evaluation of lesions demonstrated features of human xanthogranuloma. Conclusion: Rabbits fed a low-level CH diet develop sizable intraventricular masses that have similar histopathological features as human xanthogranuloma. Multiparametric MRI techniques were able to provide information about the complex composition of these lesions. J. Magn. Reson. Imaging 2016;44:673–682.",
keywords = "cholesterol, hypercholesterolemia, magnetic resonance imaging, rabbit model, xanthogranuloma",
author = "Yuanxin Chen and Hamilton, {Amanda M.} and Parkins, {Katie M.} and Wang, {Jian Xiong} and Rogers, {Kem A.} and Zeineh, {Michael M.} and Rutt, {Brian K.} and Ronald, {John A.}",
year = "2016",
month = "9",
day = "1",
doi = "10.1002/jmri.25213",
language = "English (US)",
volume = "44",
pages = "673--682",
journal = "Journal of Magnetic Resonance Imaging",
issn = "1053-1807",
publisher = "John Wiley and Sons Inc.",
number = "3",

}

TY - JOUR

T1 - MRI and histopathologic study of a novel cholesterol-fed rabbit model of xanthogranuloma

AU - Chen, Yuanxin

AU - Hamilton, Amanda M.

AU - Parkins, Katie M.

AU - Wang, Jian Xiong

AU - Rogers, Kem A.

AU - Zeineh, Michael M.

AU - Rutt, Brian K.

AU - Ronald, John A.

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Purpose: To develop a rabbit model of xanthogranuloma based on supplementation of dietary cholesterol. The aim of this study was to analyze the xanthogranulomatous lesions using magnetic resonance imaging (MRI) and histological examination. Materials and Methods: Rabbits were fed a low-level cholesterol (CH) diet (n = 10) or normal chow (n = 5) for 24 months. In vivo brain imaging was performed on a 3T MR system using fast imaging employing steady state acquisition, susceptibility-weighted imaging, spoiled gradient recalled, T1-weighted inversion recovery imaging and T1 relaxometry, PD-weighted and T2-weighted spin-echo imaging and T2 relaxometry, iterative decomposition of water and fat with echo asymmetry and least-squares estimation, ultrashort TE MRI (UTE-MRI), and T2* relaxometry. MR images were evaluated using a Likert scale for lesion presence and quantitative analysis of lesion size, ventricular volume, and T1, T2, and T2* values of lesions was performed. After imaging, brain specimens were examined using histological methods. Results: In vivo MRI revealed that 6 of 10 CH-fed rabbits developed lesions in the choroid plexus. Region-of-interest analysis showed that for CH-fed rabbits the mean lesion volume was 8.5 ± 2.6 mm3 and the volume of the lateral ventricle was significantly increased compared to controls (P < 0.01). The lesions showed significantly shorter mean T2 values (35 ± 12 msec, P < 0.001), longer mean T1 values (1581 ± 146 msec, P < 0.05), and shorter T2* values (22 ± 13 msec, P < 0.001) compared to adjacent brain structures. The ultrashort T2* components were visible using UTE-MRI. Histopathologic evaluation of lesions demonstrated features of human xanthogranuloma. Conclusion: Rabbits fed a low-level CH diet develop sizable intraventricular masses that have similar histopathological features as human xanthogranuloma. Multiparametric MRI techniques were able to provide information about the complex composition of these lesions. J. Magn. Reson. Imaging 2016;44:673–682.

AB - Purpose: To develop a rabbit model of xanthogranuloma based on supplementation of dietary cholesterol. The aim of this study was to analyze the xanthogranulomatous lesions using magnetic resonance imaging (MRI) and histological examination. Materials and Methods: Rabbits were fed a low-level cholesterol (CH) diet (n = 10) or normal chow (n = 5) for 24 months. In vivo brain imaging was performed on a 3T MR system using fast imaging employing steady state acquisition, susceptibility-weighted imaging, spoiled gradient recalled, T1-weighted inversion recovery imaging and T1 relaxometry, PD-weighted and T2-weighted spin-echo imaging and T2 relaxometry, iterative decomposition of water and fat with echo asymmetry and least-squares estimation, ultrashort TE MRI (UTE-MRI), and T2* relaxometry. MR images were evaluated using a Likert scale for lesion presence and quantitative analysis of lesion size, ventricular volume, and T1, T2, and T2* values of lesions was performed. After imaging, brain specimens were examined using histological methods. Results: In vivo MRI revealed that 6 of 10 CH-fed rabbits developed lesions in the choroid plexus. Region-of-interest analysis showed that for CH-fed rabbits the mean lesion volume was 8.5 ± 2.6 mm3 and the volume of the lateral ventricle was significantly increased compared to controls (P < 0.01). The lesions showed significantly shorter mean T2 values (35 ± 12 msec, P < 0.001), longer mean T1 values (1581 ± 146 msec, P < 0.05), and shorter T2* values (22 ± 13 msec, P < 0.001) compared to adjacent brain structures. The ultrashort T2* components were visible using UTE-MRI. Histopathologic evaluation of lesions demonstrated features of human xanthogranuloma. Conclusion: Rabbits fed a low-level CH diet develop sizable intraventricular masses that have similar histopathological features as human xanthogranuloma. Multiparametric MRI techniques were able to provide information about the complex composition of these lesions. J. Magn. Reson. Imaging 2016;44:673–682.

KW - cholesterol

KW - hypercholesterolemia

KW - magnetic resonance imaging

KW - rabbit model

KW - xanthogranuloma

UR - http://www.scopus.com/inward/record.url?scp=84982311291&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84982311291&partnerID=8YFLogxK

U2 - 10.1002/jmri.25213

DO - 10.1002/jmri.25213

M3 - Article

C2 - 26921220

AN - SCOPUS:84982311291

VL - 44

SP - 673

EP - 682

JO - Journal of Magnetic Resonance Imaging

JF - Journal of Magnetic Resonance Imaging

SN - 1053-1807

IS - 3

ER -