Abstract
Immunoassays are widely used in biochemical/clinical laboratories owing to their simplicity, speed, and sensitivity. We combined self-assembled monolayer-based immunoassays with MALDI-TOF MS to show that high-fidelity surface preparations with a novel matrix deposition/crystallization technique permits quantitative analysis of monolayer-bound antigens at picomolar detection limits. Calibration curves for intact proteins are possible over a broad concentration range and improved specificity of MS-immunoassays is highlighted by simultaneous label-free quantitation of ligand-bound protein complexes.
Original language | English (US) |
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Pages (from-to) | 3143-3146 |
Number of pages | 4 |
Journal | Proteomics |
Volume | 12 |
Issue number | 21 |
DOIs | |
State | Published - Nov 2012 |
Keywords
- Immunodetection
- MS
- Protein chips
- Protein profiling
- Quantitative analysis
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology