Mucosal lymphoid infiltrate dominates colonic pathological changes in murine experimental Shigellosis

Maria Celeste Martino, Giacomo Rossi, Irene Martini, Ivan Tattoli, Damiana Chiavolini, Armelle Phalipon, Philippe J. Sansonetti, Maria Lina Bernardini

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background. Shigella species are invasive human pathogens that cause acute rectocolitis by triggering a dysregulated inflammatory reaction in the colonic and rectal mucosa. Because mice are naturally resistant to shigellosis, there is no mouse model that mimics human disease. We explore the susceptibility of intestinal flora-depleted mice to shigellosis after intragastric infection with Shigella strains. Methods. Mice given 5 g/L streptomycin as a beverage were infected intragastrically with 1 × 108 cfu of either invasive or noninvasive Shigella strains. Results. We found that invasive Shigella strains persist up to 30 days in feces, whereas the persistence of noninvasive Shigella strains was reduced. Colonization primarily involves the colon and the cecum and, to a lesser extent, the ileum. The hallmark of inflammation in the intestinal tissue is a dramatic expansion of the lymphoid follicles, in which a high apoptotic index is recorded. Conclusions. We provide a murine model in which shigellae are able to reach their natural tissue target: the colon. Moreover, the absence of polymorphonuclear leukocyte recruitment and of epithelial cell lesions reveal some aspects of shigellosis that are usually hidden by the prevalence of this cell population. This novel model may contribute to the identification of new targets for vaccines and therapies.

Original languageEnglish (US)
Pages (from-to)136-148
Number of pages13
JournalJournal of Infectious Diseases
Volume192
Issue number1
DOIs
StatePublished - Jul 1 2005

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Bacillary Dysentery
Shigella
Colon
Proctocolitis
Introduced Species
Active Immunotherapy
Cecum
Beverages
Streptomycin
Ileum
Feces
Mucous Membrane
Neutrophils
Epithelial Cells
Inflammation
Infection
Population

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Martino, M. C., Rossi, G., Martini, I., Tattoli, I., Chiavolini, D., Phalipon, A., ... Bernardini, M. L. (2005). Mucosal lymphoid infiltrate dominates colonic pathological changes in murine experimental Shigellosis. Journal of Infectious Diseases, 192(1), 136-148. https://doi.org/10.1086/430740

Mucosal lymphoid infiltrate dominates colonic pathological changes in murine experimental Shigellosis. / Martino, Maria Celeste; Rossi, Giacomo; Martini, Irene; Tattoli, Ivan; Chiavolini, Damiana; Phalipon, Armelle; Sansonetti, Philippe J.; Bernardini, Maria Lina.

In: Journal of Infectious Diseases, Vol. 192, No. 1, 01.07.2005, p. 136-148.

Research output: Contribution to journalArticle

Martino, MC, Rossi, G, Martini, I, Tattoli, I, Chiavolini, D, Phalipon, A, Sansonetti, PJ & Bernardini, ML 2005, 'Mucosal lymphoid infiltrate dominates colonic pathological changes in murine experimental Shigellosis', Journal of Infectious Diseases, vol. 192, no. 1, pp. 136-148. https://doi.org/10.1086/430740
Martino, Maria Celeste ; Rossi, Giacomo ; Martini, Irene ; Tattoli, Ivan ; Chiavolini, Damiana ; Phalipon, Armelle ; Sansonetti, Philippe J. ; Bernardini, Maria Lina. / Mucosal lymphoid infiltrate dominates colonic pathological changes in murine experimental Shigellosis. In: Journal of Infectious Diseases. 2005 ; Vol. 192, No. 1. pp. 136-148.
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