Mucosal T cells regulate paneth and intermediate cell numbers in the small intestine of T. spiralis-infected mice

M. Kamal, D. Wakelin, A. J. Ouellette, A. Smith, D. K. Podolsky, Y. R. Mahida

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Secretions of Paneth, intermediate and goblet cells have been implicated in innate intestinal host defense. We have investigated the role of T cells in effecting alterations in small intestinal epithelial cell populations induced by infection with the nematode Trichinella spiralis. Small intestinal tissue sections from euthymic and athymic (nude) mice, and mice with combined deficiency in T-cell receptor β and δ genes [TCR(β/δ)-/-] infected orally with T. spiralis larvae, were examined by electron microscopy and after histochemical and lineage-specific immunohistochemical staining. Compared with uninfected controls, Paneth and intermediate cell numbers increased significantly in infected euthymic and nude mice but not infected TCR(β/δ)-/- mice. Transfer of mesenteric lymph node cells before infection led to an increase in Paneth and intermediate cells in TCR(β/δ)-/- mice. In infected euthymic mice, Paneth cells and intermediate cells expressed cryptdins (α-defensins) but not intestinal trefoil factor (ITF), and goblet cells expressed ITF but not cryptdins. In conclusion, a unique, likely thymic-independent population of mucosal T cells modulates innate small intestinal host defense in mice by increasing the number of Paneth and intermediate cells in response to T. spiralis infection.

Original languageEnglish (US)
Pages (from-to)117-125
Number of pages9
JournalClinical and Experimental Immunology
Volume126
Issue number1
DOIs
StatePublished - 2001

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Paneth Cells
Small Intestine
Trichinella spiralis
Cell Count
T-Lymphocytes
Nude Mice
Goblet Cells
Nematode Infections
Defensins
T-Cell Receptor Genes
Infection
Population
Larva
Electron Microscopy
Lymph Nodes
Epithelial Cells
Staining and Labeling

Keywords

  • Defensin
  • Goblet cells
  • Innate immunity

ASJC Scopus subject areas

  • Immunology

Cite this

Mucosal T cells regulate paneth and intermediate cell numbers in the small intestine of T. spiralis-infected mice. / Kamal, M.; Wakelin, D.; Ouellette, A. J.; Smith, A.; Podolsky, D. K.; Mahida, Y. R.

In: Clinical and Experimental Immunology, Vol. 126, No. 1, 2001, p. 117-125.

Research output: Contribution to journalArticle

Kamal, M. ; Wakelin, D. ; Ouellette, A. J. ; Smith, A. ; Podolsky, D. K. ; Mahida, Y. R. / Mucosal T cells regulate paneth and intermediate cell numbers in the small intestine of T. spiralis-infected mice. In: Clinical and Experimental Immunology. 2001 ; Vol. 126, No. 1. pp. 117-125.
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