Multiband spectral-spatial RF excitation for hyperpolarized [2-13C]dihydroxyacetone 13C-MR metabolism studies

Irene Marco-Rius, Peng Cao, Cornelius von Morze, Matthew Merritt, Karlos X. Moreno, Gene Yuan Chang, Michael A. Ohliger, David Pearce, John Kurhanewicz, Peder E Z Larson, Daniel B. Vigneron

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Purpose: To develop a specialized multislice, single-acquisition approach to detect the metabolites of hyperpolarized (HP) [2-13C]dihydroxyacetone (DHAc) to probe gluconeogenesis in vivo, which have a broad 144 ppm spectral range (∼4.6 kHz at 3T). A novel multiband radio-frequency (RF) excitation pulse was designed for independent flip angle control over five to six spectral-spatial (SPSP) excitation bands, each corrected for chemical shift misregistration effects. Methods: Specialized multiband SPSP RF pulses were designed, tested, and applied to investigate HP [2-13C]DHAc metabolism in kidney and liver of fasted rats with dynamic 13C-MR spectroscopy and an optimal flip angle scheme. For comparison, experiments were also performed with narrow-band slice-selective RF pulses and a sequential change of the frequency offset to cover the five frequency bands of interest. Results: The SPSP pulses provided a controllable spectral profile free of baseline distortion with improved signal to noise of the metabolite peaks, allowing for quantification of the metabolic products. We observed organ-specific differences in DHAc metabolism. There was two to five times more [2-13C]phosphoenolpyruvate and about 19 times more [2-13C]glycerol 3-phosphate in the liver than in the kidney. Conclusion: A multiband SPSP RF pulse covering a spectral range over 144 ppm enabled in vivo characterization of HP [2-13C]DHAc metabolism in rat liver and kidney. Magn Reson Med 77:1419–1428, 2017.

Original languageEnglish (US)
Pages (from-to)1419-1428
Number of pages10
JournalMagnetic resonance in medicine
Volume77
Issue number4
DOIs
StatePublished - Apr 1 2017

Keywords

  • dihydroxyacetone
  • dynamic nuclear polarization
  • hyperpolarization
  • kidney
  • liver
  • metabolic imaging
  • multiband RF pulses
  • spectral-spatial RF pulses

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Fingerprint Dive into the research topics of 'Multiband spectral-spatial RF excitation for hyperpolarized [2-<sup>13</sup>C]dihydroxyacetone <sup>13</sup>C-MR metabolism studies'. Together they form a unique fingerprint.

Cite this