Multiband spectral-spatial RF excitation for hyperpolarized [2-13C]dihydroxyacetone 13C-MR metabolism studies

Irene Marco-Rius, Peng Cao, Cornelius von Morze, Matthew Merritt, Karlos X. Moreno, Gene Yuan Chang, Michael A. Ohliger, David Pearce, John Kurhanewicz, Peder E Z Larson, Daniel B. Vigneron

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Purpose: To develop a specialized multislice, single-acquisition approach to detect the metabolites of hyperpolarized (HP) [2-13C]dihydroxyacetone (DHAc) to probe gluconeogenesis in vivo, which have a broad 144ppm spectral range (∼4.6 kHz at 3T). A novel multiband radio-frequency (RF) excitation pulse was designed for independent flip angle control over five to six spectral-spatial (SPSP) excitation bands, each corrected for chemical shift misregistration effects. Methods: Specialized multiband SPSP RF pulses were designed, tested, and applied to investigate HP [2-13C]DHAc metabolism in kidney and liver of fasted rats with dynamic 13C-MR spectroscopy and an optimal flip angle scheme. For comparison, experiments were also performed with narrow-band slice-selective RF pulses and a sequential change of the frequency offset to cover the five frequency bands of interest. Results: The SPSP pulses provided a controllable spectral profile free of baseline distortion with improved signal to noise of the metabolite peaks, allowing for quantification of the metabolic products. We observed organ-specific differences in DHAc metabolism. There was two to five times more [2-13C]phosphoenolpyruvate and about 19 times more [2-13C]glycerol 3-phosphate in the liver than in the kidney. Conclusion: A multiband SPSP RF pulse covering a spectral range over 144ppm enabled in vivo characterization of HP [2-13C]DHAc metabolism in rat liver and kidney.

Original languageEnglish (US)
JournalMagnetic Resonance in Medicine
DOIs
StateAccepted/In press - 2016

Fingerprint

Dihydroxyacetone
Radio
Kidney
Liver
Phosphoenolpyruvate
Gluconeogenesis
Noise
Magnetic Resonance Spectroscopy

Keywords

  • Dihydroxyacetone
  • Dynamic nuclear polarization
  • Hyperpolarization
  • Kidney
  • Liver
  • Metabolic imaging
  • Multiband RF pulses
  • Spectral-spatial RF pulses

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Marco-Rius, I., Cao, P., von Morze, C., Merritt, M., Moreno, K. X., Chang, G. Y., ... Vigneron, D. B. (Accepted/In press). Multiband spectral-spatial RF excitation for hyperpolarized [2-13C]dihydroxyacetone 13C-MR metabolism studies. Magnetic Resonance in Medicine. https://doi.org/10.1002/mrm.26226

Multiband spectral-spatial RF excitation for hyperpolarized [2-13C]dihydroxyacetone 13C-MR metabolism studies. / Marco-Rius, Irene; Cao, Peng; von Morze, Cornelius; Merritt, Matthew; Moreno, Karlos X.; Chang, Gene Yuan; Ohliger, Michael A.; Pearce, David; Kurhanewicz, John; Larson, Peder E Z; Vigneron, Daniel B.

In: Magnetic Resonance in Medicine, 2016.

Research output: Contribution to journalArticle

Marco-Rius, I, Cao, P, von Morze, C, Merritt, M, Moreno, KX, Chang, GY, Ohliger, MA, Pearce, D, Kurhanewicz, J, Larson, PEZ & Vigneron, DB 2016, 'Multiband spectral-spatial RF excitation for hyperpolarized [2-13C]dihydroxyacetone 13C-MR metabolism studies', Magnetic Resonance in Medicine. https://doi.org/10.1002/mrm.26226
Marco-Rius, Irene ; Cao, Peng ; von Morze, Cornelius ; Merritt, Matthew ; Moreno, Karlos X. ; Chang, Gene Yuan ; Ohliger, Michael A. ; Pearce, David ; Kurhanewicz, John ; Larson, Peder E Z ; Vigneron, Daniel B. / Multiband spectral-spatial RF excitation for hyperpolarized [2-13C]dihydroxyacetone 13C-MR metabolism studies. In: Magnetic Resonance in Medicine. 2016.
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abstract = "Purpose: To develop a specialized multislice, single-acquisition approach to detect the metabolites of hyperpolarized (HP) [2-13C]dihydroxyacetone (DHAc) to probe gluconeogenesis in vivo, which have a broad 144ppm spectral range (∼4.6 kHz at 3T). A novel multiband radio-frequency (RF) excitation pulse was designed for independent flip angle control over five to six spectral-spatial (SPSP) excitation bands, each corrected for chemical shift misregistration effects. Methods: Specialized multiband SPSP RF pulses were designed, tested, and applied to investigate HP [2-13C]DHAc metabolism in kidney and liver of fasted rats with dynamic 13C-MR spectroscopy and an optimal flip angle scheme. For comparison, experiments were also performed with narrow-band slice-selective RF pulses and a sequential change of the frequency offset to cover the five frequency bands of interest. Results: The SPSP pulses provided a controllable spectral profile free of baseline distortion with improved signal to noise of the metabolite peaks, allowing for quantification of the metabolic products. We observed organ-specific differences in DHAc metabolism. There was two to five times more [2-13C]phosphoenolpyruvate and about 19 times more [2-13C]glycerol 3-phosphate in the liver than in the kidney. Conclusion: A multiband SPSP RF pulse covering a spectral range over 144ppm enabled in vivo characterization of HP [2-13C]DHAc metabolism in rat liver and kidney.",
keywords = "Dihydroxyacetone, Dynamic nuclear polarization, Hyperpolarization, Kidney, Liver, Metabolic imaging, Multiband RF pulses, Spectral-spatial RF pulses",
author = "Irene Marco-Rius and Peng Cao and {von Morze}, Cornelius and Matthew Merritt and Moreno, {Karlos X.} and Chang, {Gene Yuan} and Ohliger, {Michael A.} and David Pearce and John Kurhanewicz and Larson, {Peder E Z} and Vigneron, {Daniel B.}",
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AU - Marco-Rius, Irene

AU - Cao, Peng

AU - von Morze, Cornelius

AU - Merritt, Matthew

AU - Moreno, Karlos X.

AU - Chang, Gene Yuan

AU - Ohliger, Michael A.

AU - Pearce, David

AU - Kurhanewicz, John

AU - Larson, Peder E Z

AU - Vigneron, Daniel B.

PY - 2016

Y1 - 2016

N2 - Purpose: To develop a specialized multislice, single-acquisition approach to detect the metabolites of hyperpolarized (HP) [2-13C]dihydroxyacetone (DHAc) to probe gluconeogenesis in vivo, which have a broad 144ppm spectral range (∼4.6 kHz at 3T). A novel multiband radio-frequency (RF) excitation pulse was designed for independent flip angle control over five to six spectral-spatial (SPSP) excitation bands, each corrected for chemical shift misregistration effects. Methods: Specialized multiband SPSP RF pulses were designed, tested, and applied to investigate HP [2-13C]DHAc metabolism in kidney and liver of fasted rats with dynamic 13C-MR spectroscopy and an optimal flip angle scheme. For comparison, experiments were also performed with narrow-band slice-selective RF pulses and a sequential change of the frequency offset to cover the five frequency bands of interest. Results: The SPSP pulses provided a controllable spectral profile free of baseline distortion with improved signal to noise of the metabolite peaks, allowing for quantification of the metabolic products. We observed organ-specific differences in DHAc metabolism. There was two to five times more [2-13C]phosphoenolpyruvate and about 19 times more [2-13C]glycerol 3-phosphate in the liver than in the kidney. Conclusion: A multiband SPSP RF pulse covering a spectral range over 144ppm enabled in vivo characterization of HP [2-13C]DHAc metabolism in rat liver and kidney.

AB - Purpose: To develop a specialized multislice, single-acquisition approach to detect the metabolites of hyperpolarized (HP) [2-13C]dihydroxyacetone (DHAc) to probe gluconeogenesis in vivo, which have a broad 144ppm spectral range (∼4.6 kHz at 3T). A novel multiband radio-frequency (RF) excitation pulse was designed for independent flip angle control over five to six spectral-spatial (SPSP) excitation bands, each corrected for chemical shift misregistration effects. Methods: Specialized multiband SPSP RF pulses were designed, tested, and applied to investigate HP [2-13C]DHAc metabolism in kidney and liver of fasted rats with dynamic 13C-MR spectroscopy and an optimal flip angle scheme. For comparison, experiments were also performed with narrow-band slice-selective RF pulses and a sequential change of the frequency offset to cover the five frequency bands of interest. Results: The SPSP pulses provided a controllable spectral profile free of baseline distortion with improved signal to noise of the metabolite peaks, allowing for quantification of the metabolic products. We observed organ-specific differences in DHAc metabolism. There was two to five times more [2-13C]phosphoenolpyruvate and about 19 times more [2-13C]glycerol 3-phosphate in the liver than in the kidney. Conclusion: A multiband SPSP RF pulse covering a spectral range over 144ppm enabled in vivo characterization of HP [2-13C]DHAc metabolism in rat liver and kidney.

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KW - Dynamic nuclear polarization

KW - Hyperpolarization

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KW - Multiband RF pulses

KW - Spectral-spatial RF pulses

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