Multicenter Prospective Study of Biomarkers for Diagnosis of Invasive Candidiasis in Children and Adolescents

Brian T. Fisher, Craig L.K. Boge, Rui Xiao, Sydney Shuster, Dawn Chin-Quee, John Allen, Shareef Shaheen, Randall Hayden, Sri Suganda, Theoklis E. Zaoutis, Yeh Chung Chang, Dwight E. Yin, Anna R. Huppler, Lara Danziger-Isakov, William J. Muller, Emmanuel Roilides, José Romero, Paul K. Sue, David Berman, Rachel L. WattierNatasha Halasa, Alice Pong, Gabriela Maron, Pere Soler-Palacin, Susan C. Hutto, Blanca E. Gonzalez, Christine M. Salvatore, Sujatha Rajan, Michael Green, Elizabeth Doby Knackstedt, Sarmistha B. Hauger, William J. Steinbach

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

BACKGROUND: Diagnosis of invasive candidiasis (IC) relies on insensitive cultures; the relative utility of fungal biomarkers in children is unclear. METHODS: This multinational observational cohort study enrolled patients aged >120 days and <18 years with concern for IC from 1 January 2015 to 26 September 2019 at 25 centers. Blood collected at onset of symptoms was tested using T2Candida, Fungitell (1→3)-β-D-glucan, Platelia Candida Antigen (Ag) Plus, and Platelia Candida Antibody (Ab) Plus assays. Operating characteristics were determined for each biomarker, and assays meeting a defined threshold considered in combination. Sterile site cultures were the reference standard. RESULTS: Five hundred participants were enrolled at 22 centers in 3 countries, and IC was diagnosed in 13 (2.6%). Thirteen additional blood specimens were collected and successfully spiked with Candida species, to achieve a 5.0% event rate. Valid T2Candida, Fungitell, Platelia Candida Ag Plus, and Platelia Candida Ab Plus assay results were available for 438, 467, 473, and 473 specimens, respectively. Operating characteristics for T2Candida were most optimal for detecting IC due to any Candida species, with results as follows: sensitivity, 80.0% (95% confidence interval, 59.3%-93.2%), specificity 97.1% (95.0%-98.5%), positive predictive value, 62.5% (43.7%-78.9%), and negative predictive value, 98.8% (97.2%-99.6%). Only T2Candida and Platelia Candida Ag Plus assays met the threshold for combination testing. Positive result for either yielded the following results: sensitivity, 86.4% (95% confidence interval, 65.1%- 97.1%); specificity, 94.7% (92.0%-96.7%); positive predictive value, 47.5% (31.5%-63.9%); and negative predictive value, 99.2% (97.7%-99.8%). CONCLUSIONS: T2Candida alone or in combination with Platelia Candida Ag Plus may be beneficial for rapid detection of Candida species in children with concern for IC. CLINICAL TRIALS REGISTRATION: NCT02220790.

Original languageEnglish (US)
Pages (from-to)248-259
Number of pages12
JournalClinical infectious diseases : an official publication of the Infectious Diseases Society of America
Volume75
Issue number2
DOIs
StatePublished - Aug 25 2022

Keywords

  • biomarkers
  • invasive candidiasis
  • Pediatrics

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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